| Objective:In the social life, the physical stress and the increasing social psychological stress such as a long time pressure leads to tension, anxiety, depression and other stimulus effect with the body has become the important factors that induce hypertension, of which incidence increase every year. Prehypertension is the precursor stage of hypertension, patients with prehypertension have been target organ damage, also have related gene changed at the same time, large amounts of studies begin to realize the importance to reverse hypertension and target organ protection by early intervention. To identify the genes of which the observation of acupuncture’s effect on stress-induced pre-hypertension rat’s heart gene expression profile, we use electric foot-shocks combined with noise to creat the moble of stress-indruced prehypertension, after 14 days, draw the heart materials with genechip and RT-PCR technology to detect, to find the related genes and their function of target organ organization change, at the molecular level to explore the biological effect of acupuncture regulate blood pressure mechanism.Methods:thirty three,9-week-old Specified pathogen free (SPF) male Wistar rats of 220±30g were randomly divided into 3 groups (n=11/group):blank control group, model control group, acupuncture control group. The model rats of stress-indruced pre-hypertension were established by electric foot-shocks combined with noise, and the cycle was 14 days. As the molding cycle beginning, the acupuncture intervene was applied on the acupuncture group rats with "Taichong" and "Quchi" point, and the intervention was given by a same person. The model control and blank control group rats were grabbed in the same restrainers for 20min without any other intervene. Blood pressure of one day before and 3 rd,5th,7th,9th, 11th,13th,15th was recorded (n=9), also like behavior change. After 14 days, draw the heart materials of all rats, use HE (2/11) and Gene chips (9/11) to detect.Result:1. In the molding cycle, the blank control group rats stay normal, as at the 3rd, systolic blood pressure of model control group elevated to 120 mmHg (P<0.01), and continuous rise to 120～139 mmHg, it means a successful modeling, also appeared behavior change. From the 5th day, acupuncture group had the lower blood pressure (P<0.05). In the modeling process, model control group were of elevated blood pressure, irritation, squealing, gnawing, hard stool, yellow urination, rough hair and bloodshot eyes.2. Microarray analysis showed that 390 gene expressions changed in molde group compared to blank group,182 were up regulated and 208 were down-regulated. Acupuncture intervention changed 321 genes,186 were up regulated and 135 were down regulated compared with the molde group (fold change≥1.5 were identified). Enriched GO terms that were significantly enriched in the gene set were response to chemical, response to estrogen, response to stimulus, positive regulation of muscle contraction, cellular process, potassium ion transmembrane transporter activity and so on. Further functional pathway analysis showed that compared with blank control group, changed genes in model control group were involved in 93 KEGG pathways (p<0.05). Compared with model control group, changed genes in acupuncture group were mainly related to 78 KEGG pathways.3. The expression of TXNIP, S100A8/A9, PDK4 mRNA along with the pressure changed, were closely related to hypertension and organ damage. Conclusion:Acupuncture at Taichong and Quchi can significantly lower the blood pressure of stress-induced pre-hypertension rats, and effects its gene expression profile in heart, the mechanism may be related to TXNIP, S100A8/A9, PDK4 mRNA expression. |
PDF Full Text Request