Study On The Effect Of Huayu Jiangzhuo Decoction On Obstructive Nephropathy By EMT Mechanism Of HK-2 Cells

research objective:1、11 culture and molecular biology techniques were used to study the effect of EMT on the morphology, molecular marker, invasion ability and the activity of TGF-beta/Smad signal pathway in hexokinase 2 (HK-2)。2、in-depth study of the biological characteristics of the treatment of obstructive nephropathy in the treatment of obstructive nephropathy, as the traditional Chinese medicine for the treatment of obstructive nephropathy can be used for reference treatment.research meaning:1、confirmed that the blood stasis soup through inhibiting TGF-β/Smad signaling pathway to reverse HK-2 cell EMT, play the role of anti renal tubular fibrosis。2、in-depth study of the biological characteristics of the treatment of obstructive nephropathy in the treatment of obstructive nephropathy, as the traditional Chinese medicine for the treatment of obstructive nephropathy can be used for reference treatment 。research method:1、The morphological changes of HK-2 cells were studied by removing stasis and turbid decoction. The appearance of cells was observed under the microscope, and the effect of EMT on HK-2 cells was judged by morphological observation.2、removing blood stasis and descending turbid Decoction on renal tubular epithelial cell invasion ability of research:different concentrations of Huayu drop turbidity Decoction in the treatment of renal tubular epithelial cells in Matrigel Transwell culture, through Matrigel in the number of cells, each the invasive ability of differences.3、For SP-1 mRNA、a-SMA mRNA、E-cadherin mRNA、Snail mRNA expression, high dose group compared with the induced group, there was significant statistical significance (P< 0.01). For E-cadherin, alpha Snail, a-SMA, FSP-1 protein expression, high dose group compared with the induced group, there was significant statistical significance (P< 0.01).4、through multi factor variance analysis, the expression of a-SMA, snail, E-cadherin, Smad2, Smad3, Smad7 mRNA, at 48h, high Chang group compared with induction group, both have statistical significance (P< 0.01), a-SMA, snail, E-cadherin, Smad2, Smad3, Smad7 egg white expression, at 48h, high Chang group compared with induction group, both have statistical significance (P< 0.01).Conclusion and significance:1 can induce HK-2 cells EMT, Huayu Jiangzhuo decoction can inhibit the occurrence of HK-2 after EMT cell proliferation, showing a dose-response relationship, experiments show that Huayu Jiangzhuo Decoction to the expression of E-cadherin, Smad2, Smad7 protein and TGF-gene increased HK-2 beta 1 induced cells, reduce alpha-SMA, Smad3, Snail protein and gene expression, three with the dose increased, the expression intensity changes, showing a dose-response relationship, while E-cadherin, α-SMA, Smad2, Smad3 alpha, Smad7 beta, Snail is the TGF-β/Smad signaling pathway in key protein, showed that Huayu Jiangzhuo decoction may be the key the TGF-β/Smad signal pathway in HK-2 cells in to inhibit or even reverse the transdifferentiation of renal tubular epithelial cells, can reverse EMT, so the tubulointerstitial fibrosis has protective effect, So as to achieve the purpose of protecting renal function.