Study On Microfluidic In Suspension Array

Biochip was an emerging technique in the field of bioinformatics and has taken great development. It realized fast measurement and analysis of biological information. Suspension array technology inherited and developed the technology of microarray. It used microsphere as the bio-materials carrier, which resulted in characteristics of large area for reaction, high throughput and sensitivity, good S/N ratio and provide us an ideal location for hybridization. The necessary operation for this technology was simple. Microfluidic device was an element from which information was collected in suspension array technology. The microfluidic system was composed of velocity control of liquid fluid, pressure driven of liquid assay and the microfluidic devices, which was an important part of the whole system.In this paper, we presented the two dimensional multi-probe sampling parallel detection of suspension array. Microsphere probes were freezingly imaged by CCD. Then all information of the suspension array to be tested was picked-up from the images. Experimental setup was designed. Its feasibility was analyzed and validated by experiment. According to theory of hydrodynamics, the flowing characteristics of microfluidic and the movement of microspheres thereof in microfluidic devices were analyzed. Microfluidic were analytically expressed through the solving of Stokes' equations. Method adopting cusped magnetic field was provided to lock microsphere probe. Microfluidic device was fabricated through method of machining, laser ablation and chemical etching and their principles were discussed detailedly. Coarseness in the microfluidic channel surface and influence of microfluidic device shape on the detection result for three fabrication method was compared.Experimental result for the microfluidic channel was provided. And the status of polystyrene micosphere in the device was given. A method for reducing background noise was presented and validated by experiment. Microsphere was freezingly imaged in microfluidic channel continuously. Some future work was prospected.