| Purpose:The study is intended to establish the immunofunction detecting net which is composed of T cell subsets, natural killer cells (NK), natural killer T(NKT), cortisol and testosterone by advanced measuring techniques——flow cytometry technique. The study aims to investigate the changes of immunofunction in athletes of beach volleyball during winter training session to find out the valuable sensitive immunofunction indexes, and detect the state of athletes during training to make the training better and better.Methods:Ten male athletes of beach volleyball participated in the winter training session with two-week training of middle workloads, two-week raining of high workloads, and one-week recovery training of middle workloads. Blood samples were collected in the adjusted period, in the 2nd, 3rd, 4th week during training and after recovery training. Blood samples were assessed by flow cytometry and other ways to determined concentrations of CD3~+, CD4~+, CD8~+, NK, NKT, testosterone and cortisol.Results:1. The variatons of immuonfunction indexes did not change simultaneously during training.2. With the increment of training volume and workloads, the expression of NKT declined.3. The T cell subsets in the 4th week during winter training session declined significantly, and it was still a low level after recovery.Conclusion:1. The NKT may be selected as the best index of evaluating immunofunction of athletes for it was very sensitive in response to training2. The consistency of testosterone and cortisol can reflect the training value and workloads effectively.3. It suggested that the immumofunction may be depressed by constant training of high workloads.
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