Effects Of "Living High-Training Low" On Anti-oxidation And Free Calcium In Mitochondrion Of Mouse's Myocardium And Skeletal Muscle

The traditional plateau training takes use of double stimulations of hypoxia environment and training under hypobaric and hypoxic envirenment, athletes will be able to endure severe stimulations to mobilize body potential, to arouse a scries of anti-hypoxia physiological and biochemical reactions. With the development of altitude training researches, the researches of basic theories under altitude training are perfectly being built up; training methods have been more improved, scientific trainings have been more enhanced.Thus some new training means and stimulant training methods have been established. In 1991, Levine first put forward "Hi-Lo", it is thought to be more effective through practice and by comparison. Hi-Lo training lets athletes live at a relatively high altitude, which fully mobilizes body to adapt to plateau hypoxia environment and digs up the body potential; as a result big burthen training can be reached when training low. This training method conquers the shortcomings when training on plateau, particularly the disadvantages about the supply of blood and oxygen and combination of proteins. Meanwhile, living in hypoxia environment can improve the ability of making use of oxygen and metabolism, especially the ability of resisting endogenous free radicals. At normal circumstance, the electron leak of mitochondrial respiratory chain is the main source of super oxygen anion, about share 95% of the reactive oxygen that produced by organism, which can be scavenged by the Mn-SOD and GSH-Px etc that line at side of mitochondrial matrix. The aim of this experiment is to observe the effects of "living high -training low" on anti-oxidation and free calcium in mitochondria of mice's myocardium and skeletal muscle, so as to discuss molecular mechanism of mitochondrial inhibition to radical oxygen species under "living high -training low" condition.32 nine-week-old male mice were randomly divided into normoxia control group (NC, n=8); normoxia training group (NT, n=8); hypoxia control group (HC, n=8); hypoxia training group (HT, n=8). The NC group takes an adaptability swimming for a week and then normal feeding without training; The HC group stays 8 hours in self-made hypoxia cabin everyday and then normal feeding at the rest of time without training; The NT set is swimming without heavy for 1 hour everyday, six days a week for 4 weeks; The HT group stays 8 hours(like HC group) in hypoxia cabin everyday, and then takes an 1 hour swimming training six days a week for 4 weekswith normal feeding.Five weeks later, all the mice take an exhausting swim and were killed at once...