Researches Into Some Mechanism In Acute Reparing Period Of Exercises And Kuanjingsan For The Closing Soft Tissues Injury

This experiment aims at looking for some faster,simpler,and more efficient means on fasting acute repairing of soft tissues damaged,reducing the tissues' adhesion and preventing the hyperplasia of scar.and improving the prognosis to the greatest extent by seeking some mechanism in acute repairing period of exercise and traditional Chinese medicine edicine Kuanjingsan for closing soft tissue damaged.For it is rubor after damaging to the closing soft tissue, getting swollen, getting hot aching and model of functiolaesa these bed solicit and there is no fracture,no skin damaged, we designed the corresponding experiment, namely the law of hot board causes little mouse's painful experiment of threshold of pain; Freun's complete adjuvant(FCA) caused the swollen experiment of big mouse's foot (resist the inflammation);The xylene caused the swollen experiment of little mouse's ear (subside a swelling ); Invigorating blood circulation silt experiment that changes the sex that the rheology appearance examines the blood and flows with the blood; Useing the high performance liquid chromatography-electrochemistry Detected (HPLC-ECD) monoamine neurotransmitters transmitter in the brain and serum; the experiment of immunohistochemistry measured the proto-oncogene C-FOS expresses. On the basise probing into the function mechanism that exercise and Kuanjingsan break up on molecular biology level and gene level repaired in acute closing damage and doing the toxic acute toxicity experiment which was designed for verifying the toxicity of Kuanjingsan,we got the following result.1 Useing model got successed: we used heavy hammer to hit to make acute closing soft tissues damaging model,which can show the pathology course very well after orgonization's damage as well as establishing the foundation for relatively testing further.2 Fitting exercise and Kuanjingsan can notably improve the threshold of pain of the little mouse that hot board caused.3 Fitting exercise and Kuanjingsan can suppress the xyol to cause little mouse's auricle swelling and good fortune to totally assist the big mouse's digit swelling that thepharmaceutical cause , there is better subsidence of a swelling that resists inflammation function.4 Fitting exercise and Kuanjingsan can improve the hemorheology of the model animal whose soft tissues were damaged and they have good function of invigorating blood circulation silt.5 Fitting exercise and Kuanjingsan can promote the soft tissue to damage little mouse's release of the single amine neurotransmitter in the brain of models,and it announces that there is a certain efficiency of easing pain in it from molecule level.6 Fitting exercise and Kuanjingsan can reduce soft tissue damage model old mouse single amine content of neurotransmitter in the serum obviously to come loose, the endogenous release which stimulates the thing, help to damage the repair organizing after reducing and organizing and damaging.7 Fitting exercise and Kuanjingsan can suppress the soft tissue to damage big mouse's centre of models and periphery C-FOS gene expression effectively, which announces that they can ease pain very well from the gene level.