The Effect Of Simulated Alternating Hypoxia Training Of Gradient Altitude To Physiological And Biochemical Indexes Of Liver In Rats

Experimental purpose: using Artificial low pressure chamber simulating altitude training method, establishing the alternate elevation gradient animal training model anchored in Lanzhou 1500m above sea level, this thesis tries to discuss the effect of the low oxygen training on the fixed altitude of 2500m, 4000m and 2500m 4000m alternately above sea level on the liver antioxidant capacity, anti-injury capacity, and indicators of hepatic energy metabolism, c-fos gene expression in order for the athletes training with high high alternating reference.Experimental Methods: in this experiment, healthy male Wister rats were studied to establish animal training model with Treadmill training methods., the rats, which were adapted to treadmill training were divided into screening: Normoxic quiet group (NQ), Normoxic training group (NT), altitude of 2,500 meters hypoxia training group (2500T), altitude of 4,000 meters hypoxia training group (4000T), altitude of 2,500 meters and 4,000 meters alternating hypoxia group (AHT). Exercise group (NT, 2500T, AHT, 4000T) rats, and were trained for 4 weeks, 6 days a week, resting 1 day by incremental continuous. After two weeks of exercise training, the training group were randomly selected six, and respectively, let them run to death in a treadmill with the speed of 25m/min. And then decapitated immediately, took out the liver, fixed in formalin and used to detect c-fos expression in the liver. After completion of four weeks training, the other rats were trained four days to recovery in Plain, and in 5 days, let them run to death exhaustively with a treadmill speed of 25m/min, then decapitated immediately, and recorded the exhaustive time. Then the rats were measured liver enzymology, MDA content, lever SOD, GSH-PX, CAT, MDA, SDH, ATP enzyme, c-fos gene expression, blood BUN, ALT, AST indicators of different changes. NQ rats did not participate in sports, were decapitated together with the rat under the training of four times, and took the liver out instantly to detect c-fos gene expression in the liver, as controls.The experimental results show that:(1) The alternating hypoxic training can significantly prolong the treadmill time to exhaustion and improve the endurance(2) The alternating hypoxia training's rat lever GSH-PX, CAT is higher than others which shows that the alternating hypoxia training rat is more effective in removing free radical; the rat lever MDA of the hypoxia groups is higher than normoxia group, which is possibly because of hypoxia and exercise had a synergistic effect on the liver peroxidation damage of free substrate.(3) The enzymes activities of ALT, AST in rat blood of the hypoxia groups is lower than normoxia training group, indicating that the ability of liver to resistance injury is improved.(4) The alternating hypoxia training can effectively improve the enzyme activity of Na⁺ -K⁺-ATP, Ca²⁺-ATP, SDH of the rat liver which indicates that the lever aerobic metabolism ability is improved.(5) The alternating hypoxia training can effectively improve the liver glycogen reservation, and provide more energy for sports.(6) There is no difference in c-fos gene expression in the liver of the rat of two weeks trainnig, while this of four weeks trianing decresed, and long alternating hypoxic training c-fos gene expression in the liver is lower than others. It may because c-fos gene expression in the liver are related to the increasing lever anti-stress ability.Conclusion:After around simulation gradient hypoxic training at an altitude of alternate resume training after 4 days often oxygen again, moderate intensity exercise to exhaustion indicators suggest that each rat analysis, simulation 2500m 4000m alternate at an altitude of hypoxic training altitude and load relatively appropriate could evidently increase the rat liver GSH-PX, CAT,Na⁺-K⁺-ATP,Ca²⁺-ATP,SDH enzyme activity and liver glycogen reserves than other groups, and significantly reduced blood ALT, AST enzyme activity levels and liver cell c-fos expression.Thus promotes, to some degree, antioxidation, anti-damage and the ability of aerobic metabolism, effectively improve the rat liver of moderate intensity exercise of adaptability.