Effects Of Endurance Exercise On The P53/SCO1/SCO2 Gene Expression Of The Different-Months-Old ICR Mice

A gene known as guard the p53,in core location of multiple cellular signal transduction pathways,p53 gene(also known as 'tumor suppressor genes')is specifically employed to regulate energy metabolization, check the cell cycle, balance multiple signaling paths, keep and maintain oxidative stress signals in a steady state. Keeping p53 gene in a steady state can prevent formation of cancerous cells and delay senility. At present, the topic concerning tumor and aging has drawn great public attention around the world, more and more people has acknowledged the importance of related topic. After decades of efforts, scientists has made tremendous contributions on issues of both tumor and aging. Cancer and aging are complementary and mutually affected each other. Early-aging of organs osteoporosis are the results of long-term defense against cancer gene. One's longevity and health can be improved if the method of balancing cancer prevention and aging deferment can be found. P53 is the critical point of balancing cancer and aging. Some scientists found that appropriate physical exercise can effectively promote the body's metabolism, which can reduce the probability of cellular canceration and can slow cell aging process. Appropriate exercises can make p53 to regulate energy metabolization signaling pathways, thus maintaining the signal of p53 gene in a steady-state. P53 genes can transactivate SCO2, thus further activating mitochondrial respiration. SCO2 play a crucial role in integration of COX II into synthesis of Cytochromes C oxidase. The depletion of p53 and SCO2 might lead to the shift from the mitochondrial aerobic respiration to glycolysis. The experiments focused on the changes in gene expression of p53, SCO1 and SCO2 in skeletal muscle. Discussing the pathways of enegy metabolization and explain how exercise regulates these pathways to improve the body's capacity of oxidative respiration.Objection:This report investigates the effects of endurance exercise on the p53/SCO1/SCO2 of skeletal muscle of the different-months-old ICR Mice.Methods:32 mice are fed for a week in an adaptive laboratory, and then 2-months-old mice were randomly divided into 2 groups - quiet group (YC, n=8) and endurance training group (YE, n=8).10-months-old mice were also divided into 2 groups - quiet groups (OC, n=8) and endurance training group (OE, n=8). Endurance training group will be trained for 4 weeks on an animal treadmill refitted by Joe Hill, and endurance trainings are conducted on a daily basis from 18:00 to 19:00 except for Sunday. After four weeks of training, all tested mice were killed due to the broken neck. The gene transcription level of p53, SCO1 and SCO2 can be determined by time fluorescent quantitative PCR.Results:After four weeks of endurance training on a treadmill, by comparing 2-months-old YE mice and 2-months-old YC mice, p53 and SCO2 gene expression shows significant difference, whereas SCO2 shows highly significant difference. However, when compared 10-months-old YE mice with 10-months-old YC mice, p53 gene expression indicates no significant difference, whereas the magnitude of SCO1 gene expression increases in a negative trend and the magnitude of SCO2 gene expression shows significant difference.Conclusions:For 4-weeks trained 2-month-old mice, there is certain increase in magnitude of P53 SCO2 gene expression, the magnitude of SOC1 gene expression increases considerably. It can be also observed that there is no relation between the magnitude of p53 gene expression and ageing effect, whereas the magnitude of SOC1 gene expression and ageing effect are in inverse proportion. As for 4-weeks trained 10-months-old mice, the results shows an evident rising tendency in the magnitude of SCO2 gene expression, indicating that p53 can effectively maintain mice body in a steady state and balance genetic integration. On the other hand, the results of SCO1 and SCO2 gene expression, it can be observed that endurance training can produce good sport adaptability and effectively facilitate aerobic respiratory effect, thus obtaining expected results.