Determination Of Ointment Beta, Beta The'-Dimethyl Acrylyl Aka Content And Pharmacodynamic Studys On The Compound Prescription Purple Grass Scalds

Burns is caused by heat, light, electricity, chemicals and radiation, it is a very complicated traumatic disease, usually appear the organization and mucosa damage in skin and muscle. The burn, the skin is damaged, outer defensive barriers dermis and muscle tissue inflammation, produced by local microcirculation, capillary dilate, permeability, produce skin irritation. Existing treatments for burn medicine is antiphlogistic, anti-infection, accelerate wound healing, promote skin grows. currently antibiotic medicines is more used , but not only excessive antibiotic resistant to human body, still can kill some beneficial bacterium inside human body. Chinese medicine therapy and burn mainly adopts external form, therefore, traditional Chinese medicine treatment showed obvious advantage by its small side burn .Lithospermum Arnebia euchroma (Royle) Johnst, which belongs to Boraginaceae perennial herb, is a commonly used traditional Chinese medicine, with the effect of cooling blood, enhancing blood circulation and detoxification. . It is a commonly used drug for water and fire burns. Scalded by hot liquids (such as boiling water, hot oil), high temperature solid (hot metal, etc.) or as high-temperature steam and other damage caused by burns. Burn as frequently occurring in daily life, common diseases, and traditional medicine for the treatment of burns has been for many years. The topics to Fufang zicao burn ointment as a starting point to find a new drug treatment of burns. Successful development of this project will have broad market prospects and social significance.In this paper, the literature review and experimental part of Fufang zicao burn ointment was studied.1 Literature review of medical understanding and treatment of burns, the research progress in three areas Lithospermum.2 Experimental Medicine2.1 The Determination ofβ,β'-dimethylacrylalkani in Fufang zicao burn ointment . Column using Agilent SB; acetonitrile - water - methanol (700:300:0.5) as mobile phase; detection wavelength 275nm; of compound shikonin burn ointmentβ,β'-dimethyl acrylamide content were Aka Ning measured results show that the 40.6-365.4ng good linear relationship within the precision, reproducibility and stability of 8 hours are in line with the national drug standards.2.2 Pharmacodynamic results show that the main compound shikonin burn ointment in treating burns effect. According to research purposes, the proposed use of rats, rabbits, soft tissue injury test, xylene, carrageenan inflammation test, hot plate, acetic acid pain test, micro-cycle test of compound shikonin the main pharmacological effects of burn ointment, is the main product provide experimental evidence for pharmacological treatment burns.2.2.1 rats, rabbits experimental soft tissue injury: burn ointment compound shikonin low, medium and high dose groups in the injury symptom index, histological analysis, swelling, inflammation, and Vaseline were the factors of significant difference between group ; can reduce the damaged tissue bleeding, blood stasis, promote the absorption of hematoma and ecchymosis dissipation; significantly reduce the damage of local muscle fiber swelling, degeneration and necrosis, inflammatory cell infiltration, and promote injury of local muscle fiber regeneration; significantly reduce the damage limb volume; significantly lower inflammatory factors in local tissue IL-1, IL-6, TNF, TXB2, the concentration, reduced TXB2/6-keto-PGF1αratio increased 6-keto-PGF1αconcentration.2.2.2 anti-inflammatory test: burn ointment compound shikonin low, medium and high dose group compared with the control group, p-xylene, carrageenan-induced inflammation was significantly inhibited (P <0.01);2.2.3 the analgesic test: burn ointment compound shikonin low, medium and high dose group compared with the control group, on the hot plate stimulation, chemical stimulation of the pain, the pain threshold test has reduced the role of (P <0.01);2.2.4 micro-cycle test: 5,10,20,30 min after administration of burn ointment compound shikonin high, medium and low-dose group, venous and arterial diameter increases, increase the number of capillaries open with the Vaseline was significant compared differences (P <0.01).Conclusion: Fufang zicao ointment has anti-burn soft tissue damage, pain, inflammation and the role of promoting blood circulation.