Expression And Clilical Significance Of Chemokine Receptor CCR7 In Colorectal Cancer

Objective To determine the expression status of chemokine receptor CCR7 and to explore the pathogenesis of clinical significance in colorectal cancer. In order to understand CCR7 expression in colorectal cancer can predict lymph node metastasis.Methods Collected 56 cases of clear clinical and pathological diagnosis of colorectal cancer specimens, including 27 cases of colon cancer, rectal cancer in 29 cases. All specimens will be made into two, one placed in liquid nitrogen cryopreservation, and another one were fixed with formaldehyde. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of CCR7mRNA in specimens with colorectal cancer which placed in liquid nitrogen cryopreservation. Immunohistochemistry was used to detect CCR7 protein in specimens with colorectal cancer which were fixed with formaldehyde.Results There are 31 cases of colorectal cancer specimens which the CCR7 mRNA were positiveiy expressed in 56 cases (55.3%). In their matching adjacent normal tissue corresponding to only 7 patients (12.5%) were detected positive expression of CCR7 , 38 cases (67.8%) colorectal cancer specimens detected the expression of CCR7 protein, 6 cases in which strong positive, In the corresponding adjacent normal tissue, there are 9 cases (16.1%) CCR7 expression. The expression level of the protein of CCR7 in lymphatic metastasis tissues was significantly higher than that in non-lymphatic metastasis tissues. Conclusion 1. CCR7 overexpression in colorectal cancer, CCR7 expression may be associated with progression and metastasis of colorectal cancer was closely related.2. The examinatian of CCR7 may be useful in predicting lymph node metastasis of colorectal cancer.