Expression And Signification Of Pulmonary Surfactant-associated Protein-a In The Vaginal Fluid Of Vulvovaginal Candidiasis

Vulvovaginal candidiasis(vvc) is a kind of vulvovaginal inflammation caused by candida infection,which is a a common disorder in women of childbearing age. The infection affects approximately 75% of women at least once during their lives, most frequently young women of childbearing age. 5–8% of adult women have recurrent vulvovaginal candidosis. Patients,suffering of at least four episodes of candida infection during 1 year,have a recurrent infection and are therefore recognized and classified as recurring vulvovaginal candidosis (RVVC) patients.With the developments of the researches in pathogenic mechanisms, now it is believed that the recurrence have obvious relevance to the deficiency of vaginal-associated immunity.Recent studies have focused on the importance of the innate immune factors in the vaginal host defense mechanism against mucosal candidal infections.Research shows pulmonary surfactant-associated protein A(SP-A) is a protein produced by squamous epithelium,namely the vaginal mucosa,which have important immunoregulation function.The expression of SP-A in the vaginal fluid might contribute to the recurrence of RVVC.Objective:It was to study the expression and signification of pulmonary surfactant-associated protein-A in the vaginal fluid of recurrent vulvovaginal candidiasis(RVVC).Methods:Human vaginal fluid from 28 RVVC patients,30 VVC patients and 30 healthy women(the control group)were collected, respectively.Enzyme-linked immunosorbent assay(ELISA) was applied to detect the levels of SP-A protein.The expression of SP-A gene was detected by reverse transcription–polymerase chain reaction (RT–PCR).Results:The levels and of SP-A of VVC group were higher than that of the control group ( P < 0. 05).The levels of SP-A of recurrent vulvovaginal candidiasis (RVVC) group were higher than those of VVC group and control group.The expression of SP-A mRNA in VVC was higher than that in the control group.The expression of SP-A mRNA in RVVC was significantly higher than those of VVC group and control group.Conclusion:SP-A might be an essential component in RVVC resistance. The qualitative and quantitative alterations of normal SP-A may play a role in the pathogenesis of RVVC.