MiR-206 Inhibits CDC42 Protein Expression, Invasion And Migration Of MDA-MB-231 Cells

Objective:To investigate whether miR-206 could regulate Cdc42 protein expression and cytoskeleton remodeling.Methods:miR-206 was transfected into MDA-MB-231 cells using LipofectamineTM 2000, after 48h cells were harvested. Western blot was used to detect proteins expression, Actin-Tracker Green was used to stain F-actin and filopodia was observed under fluorescent microscope. Invasion Assay and Migration Assay were taken to examine the invasive and migratory ability of cells respectively.Results:Protein expressions of Cdc42, MMP-2 and MMP-9 were down-regulated (P<0.01). The number of filopodia in blank group were (14.99±5.53) per cell, EGF group were (23.59±3.92), miR-206 group were (9.45±3.59) and miR-206+EGF group were (11.77±2.85). The filopodia number in miR-206 and miR206+EGF group were much less than which in the blank group or EGF group (P<0.05). The invasion and migration of miR-206 transfected MDA-MB-231 cells were significantly lower than MDA-MB-231 cells [Invasive cells: blank (311.7±23.5) , miR-206 (65.0±13.9), P<0.05; migratory cells: blank (793.0±76.3), miR-206 (415.3±20.0), P<0.05].Conclusion:miR-206 down-regulates Cdc42 protein expression of MDA-MB-231 cells, and inhibits the formation of filopodia, and can inhibit the invasion and migration efficiency in MDA-MB-231 cells.