| Objective:To investigate the differences on osteopontin expression (OPN) of thoracic aorta between renovascular hypertensic rats and normol rats, then discuss the relation of OPN and vascular remodeling in hypertension, and reaserch the influence of the atorvastain calcium on the expression of OPN and vascular renmodeling in renovascular hypertensic rats in the end.Methods: The 2-kindey,1-clip hypertensive rats were prepared with male (Sprague-Dawlay)SD rat. Twenty-one rats were randomized into sham group(n=7), hypertension group(n=7) and statins group(n=7). Statins group was treated with atorvastatin calcium 30mg/(kg.d) four weeks after model construction and lasted 8 consecutive weeks, given through lavage. All rats were evaluated caudal systolic blood pressure(SBP) and blood lipid, microscopic changes in vascular structure and ratio of intima media thickness to lumen diameter(MT/LD) of thoracic aorta. Expressions of OPN were assessed with immunohistochemical staining, expressions of OPN mRNA with Real-time quantitative PCR. Results: Compared with sham group, hypertensive group showed significant increase in SBP(161±8mmgh vs 112±6mmgh, P<0.05),MT/LD(0.067±0.005 vs 0.029±0.005, P<0.05) and the expression of OPN(0.24±0.07 vs 0.10±0.06, P<0.05) and OPN mRNA(0.1435±0.0434 vs 0.0554±0.0235, P<0.05), and significant decrease in TC,LDL-c and HDL-c level(P<0.05). As compared with hypertensive group, MT/LD(0.036±0.005 vs 0.067±0.005, P<0.05) and the expression of OPN(0.18±0.09 vs 0.24±0.07, P<0.05) and OPN mRNA(0.0994±0.0293 vs 0.1435±0.0434) of statins group were significantly decreased, HDL-c level significantly increased(P<0.05) , TC,LDL-c level significantly decreased(P<0.05).Conclusions: (1) The significantly increased OPN expression of the thoracic aorta may be related to the development of hypertensive vascular remodeling.(2)Atorvastatin calcium was shown to improve the development of vascular remodeling in rats, possibly associated with decreased expression of OPN.
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