Screening Indentification And Fermentation Optimize Of The Bacterium Produceing Cholest-4-en-3-one

In recent years,steroidal compounds as the second medicine only to antibiotic drugs,has been widely applied to the area of medical treatment.It has been widely used to treat rheumatism,cardiovascular, collagen disease, lymphatic leukemia, organ transplantation, anti-tumor, bacterial encephalitis, skin diseases, endocrine disorders, age-related diseases and so on.Cholest-4-en-3-one,as cholesterol oxidation products,could be slimming food additives or anti-hyperlipidemic drugs which can inhibits the accumulation of body fat, prevent the occurrence of obesity and the corresponding symptoms.It is valuable in the treatment of liver disease and the prevention of resistance to aging of the skin keratinocytes.At the same time,it is the important raw material of some synthetic hormone drugs.The methods of synthetic are chemical synthesis and microbial transformation.First, from the soil samples gathered,filter strain which can turn cholesterol into Cholest-4-en-3-one with better output.Determine the strain is Fusarium sp, maybe Fusarium oxysporum by the analysis of physiological and biochemical identification and 18SrDNA Gene Sequences.Then, crystallization of the product purified from fermentation products were Confirmed is derived from cholest-4-en-3-one and purity of 99.8% after MS, NMR, IR, melting point test. Last, optimizing fermentation medium, it's found that Carbon has the greatest effect on Cholest-4-en-3-one 's production, and the Nitrogen and Metal ions next. Optimization of fermentation conditions to the best fermentation conditions for the temperature 30℃, pH7.0, then all the age of 36h, inoculum size 7.5%, shaker speed 200, fermentation time of 84h.