Expression And Significance Of 14-3-3 Proteins In Human Primary Liver Carcinoma Tissue

Background and Objective: 14-3-3 protein is a group of highly conservative acid protein family by different genes encoding, there are seven subtypes in mammals, includingβ,γ,ε,η,σ,θandζ.The expression of 14-3-3 proteins in specific tissues is isoform-specific.14-3-3 proteins can interact with more than 300 target proteins in a phosphorylation-dependent and phosphorylation-independent.14-3-3 proteins play an important role in many cellular processes ,such as cell cycle,cell signal transduction and apoptosis,and were showed a close associate with the cancer development and progression. In the present study, the expression of 14-3-3 isoforms mRNA and proteins were observed in human primary hepatic carcinoma and in human normal liver tissues,and explored the correlation and significance between 14-3-3 proteins expression level and human primary liver carcinoma .Methods: Sixteen human primary hepatic carcinoma samples and ten tumor-free liver tissues obtained from the hepatectomy.The levels of all seven 14-3-3 isoforms mRNA were examined by RT-PCR,and after statistical analysis, the protein expression were identified by Western Blotting to those isoforms which mRNA expression are different.Results: A basal expression of all the seven 14-3-3 isoforms mRNA were detected by RT-PCR both in the liver cancer tissues and the non-cancer ones.However the expression level of 14-3-3βandηmRNA were significantly increased in hepatic carcinoma tissues by comparing with the tumor-free tissues(P<0.01).The protein expression level of 14-3-3βandηwere also significantly increased in hepatic carcinoma tissues by comparing with the tumor-free tissues by Western Blotting(P<0.01).Conclusions:1. There was a basal expression of all the seven 14-3-3 isoforms mRNA both in human primary liver carcinoma and tumor-free tissues, which may be required to maintain normal liver function.2. The mRNA and protein expression of 14-3-3βandηin hepatic carcinoma tissues were up-regulation,which suggested those two isoforms of 14-3-3 proteins may be involved in the development of the human primary liver carcinoma.