The Development Of Analytic Method And Metabolized Analysis Of Candida Albicans Biofilm

As it's quickly developed, metabolomic has been widely used in the fields of microbiology, botany, disease diagnosis and so on. Our study is about the changing of metabolic of Candia albicans and the cause of this changing. We use the technical platfolm of GC-MS, HPLC-MS.Candida albicans is the most commonly associated dimorphic fungus of humans in the Candida species. It can exist as either a commensal or opportunistic pathogen, causing superficial and invasive infection. Because of its transition from yeast to hyphal growth and unavailable treatment of these infections, more and more drug resistance was reported in the past few years. The formation of biofilms increases the risk of infection and has a high level of drug resistance. The progression of a mature biofilm is dependent on cell adhesion, extracellular matrix production, and the yeast-to-hypha transition in C. albicans. Many studies are about how to preventing the formation of biofilms, it found that the transition from yeast to hyphal growth can be induced by various conditions.Farnesol, an extracellular quorum-sensing molecule, can inhibit the induction of hyphal growth and biofilm formation in C. albicans. Farnesol can also be cytotoxic at certain concentrations. It's a potential desire to cure C. albicans infection or at least to supply reference about the study of drug resistance of C. albicans.Trehalose, a disaccharide of glucose (a-D-glucopyranosyl a-D-glucopyranoside), has been consistently proven as a rapid mechanism of cell protection against a variety of stresses. It can be used as storage carbon source. Here, we reported the changing concentration of trehalose in C.albicans biofilms with or without intervention by adding farnesol in the culture. The result indicates that the concentration of trehalose changes with intervention by adding farnesol. In order to explain the changes of trehalose, we study the transcription level of genes in the metabolism cycle. These genes are hxk,tps1,tps2,ath1,nth,zwf. It is clear that after intervention by farnesol, the level of transcription changes obviously.In this study, we developed methods to determine trehalose in C.albicans respectively, and try to explain the changing concentration of trehalose.