To Investigate Antioxidants On Human Vascular Endothelial Cells Inflammatory Response Of The Intervention

Objective:Induced by lipopolysaccharide in human umbilical vein endothelial cell to produce inflammatory response simulation of ALI in the lung microvascular endothelial cells in inflammation, and applied antioxidants to interfere with the inflammation in the early time. To investigate the antioxidants on the intervention effect and experimental data from antioxidant drugs treat ALI.Methods:The human umbilical vein endothelial cells were studied, LPS induced the inflammatory response, and intervened by antioxidants. The Experiments cell were divided into four groups, A group: normal control group; B group: LPS model group; C group: GSH intervention group (GSH group); D group: Edaravone group. Observed at three time points, including 2 hours, 6 hours, 12 hours, and set up 6 holes at each stage. cells morphology and growth situation observed by inverted microscope, tumor necrosis factor-α, interleukin-8 and 8-isoprostane in cell supernatant tested by enzyme immunoassay (ELISA); cell apoptosis observed by DAPI staining; and the cell apoptosis rate tested by using flow cytometry. Then the measured values of each index were analyzed.Results:1. the levels of 8-isoprostane of LPS model group were significantly higher than normal control group at each observation time point(P<0.01). 8-isoprostane levels of Intervention group were lower than LPS group (P <0.01) at each observation time point; but higher than the normal group (P <0.01).2. the levels of TNF-αand IL-8 of LPS model group were significantly higher than normal control group at each observation time point(P<0.01). TNF-αand IL-8 levels of Intervention group were lower than LPS group (P <0.01) at each observation time point; but higher than the normal group (P <0.01).3. There was a linear relationship between 8-isoprostane concentration and vascular endothelial cell apoptosis rate,inflammatory cytokines and the inflammatory cytokines;4 The apoptotic cell were observed in LPS model group; The apoptotic peak were revealed by the flow cytometry. The apoptosis rate of LPS model group were significantly higher than normal control group at each observation time point(P <0.01). The apoptosis rate of Intervention group were lower than LPS (P <0.01) at each observation time point, but higher than the normal group (P <0.05).Conclusion:1 This study successfully induced inflammatory response in vitro cells, which will contribute to the further study of the ALI;2 LPS can induce apoptosis of vascular endothelial cells, pulmonary microvascular endothelial cell apoptosis might be one of the mechanisms of acute lung injury, so inhibition of vascular endothelial cell apoptosis can be used as a target for treatment of ALI;3. Anti-oxidants can reduce oxidative stress during ALI, it can make strong inhibition to lung inflammation and vascular permeability in ALI; It also can reverse the oxidant /antioxidant imbalance rapidly, antioxidants may be a new drug options of ALI.