Apelin/APJ System Involves In The Pathogenesis Of Human Lung Cancer

Aim:1. To study the relationship between apelin/APJ system and human lung cancer;2. To observe the effect of apelin on the proliferation of human lung adenocarcinoma cell line A549.Methods:1. The distribution of APJ in human tumor tissues was detected by Immunohistochemistry staining;2. The plasma apelin level in tumor patients was detected by ELISA;3. The distribution of APJ in human lung adenocarcinoma cell line A549 was detected by Immunofluorescence;4. The expression of APJ in human lung adenocarcinoma cell line A549, human gastric adenocarcinoma cell line BGC823 and liver cancer tiusse was detected by Western blot;5. The effect of apelin-13 on the proliferation human lung adenocarcinoma cell line A549 was detected by CCK-8 assay.Results:1. The expression of APJ in cancer cells of lung adenocarcinoma,lung squamous cell carcinoma,hysteromyoma,liver cancer,gastric cancer,cervical carcinoma was higher than noncancerous tissues;2. The plasma apelin level in lung cancer patients,cervical cancer patients and gastric cancer patients was higher than normal persons;3. The APJ expressed on the membrane and cytoplasma of human adenocarcinoma cell line A549;4. The APJ expressed in lung adenocarcinoma cell line A549, gastric adenocarcinoma cell line BCG823 and liver cancer tissue. The expression of APJ in A549 was higer than BGC823 and liver cancer tissue; 5. Apelin-13 promoted the proliferation of A549 cell in concentration and time dependent manner.Conclusion:The expression of APJ in cancer cells of lung adenocarcinoma, lung squamous cell carcinoma, hysteromyoma, liver cancer, gastric cancer, cervical carcinoma was higher than noncancerous tissues. Apelin could promote the proliferation of human lung adenocarcinoma cell line A549. Apelin/APJ was probably involved in the pathogenesis of human lung cancer and other tumors.