Houttuynia Treatment Of Cytomegalovirus Infection In Pregnant Women In Clinical And Experimental Study

Research topicsHouttuynia treatment of cytomegalovirus infection in pregnant women in clinical and experimental study.The objective of the studyTo study traditional Chinese medicine treatment of human cytomegalovirus Houttuynia (HCMV) Infection. The use of high performance liquid chromatography (HPLC) determination of clinical treatment of maternal HCMV infection in anti-viral active ingredient Houttuynia cordata Thunb content of quercetin. To investigate in vitro antiviral activity of quercetin from Houttuynia cordata Thunb on human cytomegalovirus and its mechanism.Study DesignSelected in December 2007-2009 years in June, Huazhong University of Science and Technology in Medicine, Tongji Medical College Hospital (Wuhan City No.1 Hospital) Obstetrics and Gynecology 1900 cases of out-patient screening of pregnant women, detection of serum human cytomegalovirus HCMV-IgG, IgM, found that HCMV-IgM positive in 60 cases of the study. Group to give Houttuynia Jinye Baidu Granule decoction and treatment 14 days later, the review HCMV-IgM negative rate. Then the use of high-performance liquid chromatography determination of anti-viral active ingredient Houttuynia quercetin content, and method of using cell culture experiments of quercetin in vitro anti-human cytomegalovirus (HCMV) role and its mechanism.The treatment and observation methods1. The HCMV-IgM positive in 60 cases of random number table groups:control group 30 cases, the treatment group 30 cases. The control group Jinye Baidu Granule (Alliance Pharmaceutical Group Co., Ltd. Wuhan production, composition:Daqingye, dandelion, honeysuckle, Houttuynia, bag 10g) treatment,3 times a day, each one bag; treatment group Houttuynia herb 30g, from the First Hospital of Wuhan.City Room unified Jian Zhi decoction is made bag 200ml. a bag of oral, twice daily, are taking 14 days.1 week after withdrawal elbow sterile venous blood collected 4 ml, the serum stored at -20℃, review HCMV-IgM negative rate.2. Observed after treatment, negative conversion rate of HCMV-IgM positive, using EL ISA assay, reagents HCMV-IgG, IgM, EL ISA kit purchased from the U.S. Hope Corporation.3. HPLC determination of anti-viral active ingredient Houttuynia Determination of Quercetin:The Agilent ZORBAX ODS column with methanol -0.4% phosphoric acid (52:48) as mobile phase; 368nm detection wavelength, flow rate:1.0ml.min-1, column temperature:30℃.4. To use anti-virus and effective part of Houttuynia dubbed in different concentrations of quercetin solution, methyl thiazolyl tetrazolium assay (MTT) detection of cell toxicity, quercetin solutions of different concentrations were applied to be HCMV infected cells, by observing the cell cytopathic effect (CPE), MTT assay Cell viability was to explore the effect of quercetin on HCMV infected cells and therapeutic effects of HCMV in the cell proliferation inhibition and prevention role, thus the mechanism, of its anti-virus research.Statistical MethodsClinical observation of measurement information and data are entered SPSS 13.0 software for t test and x2 test.Cell culture experiments the data to mean±standard deviation that the use of statistical software analysis SPSS13.0Results1. The control group before and after treatment HCMV-IgM negative conversion rate73.3%, P<0.01, the difference was statistically significant. The treatment group before and after treatment HCMV-IgM negative conversion rate70.0%, P<0.01, the difference was statistically significant. The treatment group compared with the control group, u=0.2837, P> 0.05, difference was not significant.2. HPLC determination of Houttuynia determination of anti-viral active ingredient content of quercetin:The linear range of 0.24-1.20μg, the correlation coefficient r=0.9973, average recovery was 98.75%, RSD=0.66%.3. Quercetin half of the toxic concentration of (TC50) 4.75mg/ml, pairs of HCMV in the cell proliferation and prevention of the role of the therapeutic effect of half of the effective concentration (EC50) were 0.30 mg/ml and 0.25 mg/ml, treatment index for the (TI) 15.83.ConclusionCytomegalovirus infection in pregnant women after natural seroconversion rate of about 40%, this will lead to the fetus in utero and through the birth canal HCMV infection, resulting in pregnant women, miscarriage, premature delivery, fetal malformation and birth defects and other serious consequences. Chinese medicine Houttuynia cordata Thunb treatment of pregnancy a woman cytomegalovirus infection significant effect on the infected HCMV-IgM negative conversion rate was increased, and the right of pregnant women and fetal side effects is small, worthy to be popularized.Houttuynia anti-viral active ingredients of quercetin could inhibit human cytomegalovirus infection cytopathic effect, CPE inhibition rate increased with increased drug concentration.