Association Of MMP-9 And Hl Polymorphisms With Cerebral Infarction

Object: To study whether the single nucletide polymorphisms(SNPs) of matrix metalloproteinase-9 (MMP-9)gene and hepatic lipase (HL) gene are associated with cerebral infarction(CI) in Chinese,and furtherly discuss the mechanisms of the occurance of CI.Method: Four mutations respectively at 279 fromR to Q,-1562 from C to T of MMP-9 gene and -514 from C to T,-763fromA to G of HL gene were determined in 100 patients with CI,72 contrals from the population in the epidemic survey by a combination of polymerase chain reaction(PCR) and restriction fragament length polymorphism(RFLP). Association study was performed to analyze the allele and gene frequency distribution and genetic comparison of combined effects of correlation analysis and CI on the basis ofthe data from statistical software package SPSS13.0Result:1.The gene distribution of Hardy-Weinber genetic equilibrium test (P> 0.05) consistent with the genetic balance, a group representative.2.MMP-9 gene R279Q polymorphism genotype (P = 0.047) and allele frequency (P = 0.016) distribution has significant difference between CI and healthy controls. R allele frequency was significantly higher than healthy controls (74%, 61.8%), may be related to CI There were no statistical significance of distribution of MMP-9 gene C-1562T and the HL gene C-514T, A-763G genotype (P> 0.05) and allele frequencies (P> 0.05) .3.There were statistical significance of distribution of HL C-514T T allele and MMP-9 R279Q combinated genes between between CI and healthy controls (χ2 = 4.718, p = 0.03),,and the distribution of HL A-763G G allele and MMP-9 R279Q combinated genes were also a significant difference (χ2 = 3.870, p = 0.049). there were no statistical significance of distribution of HL C-514T C allele ,HL A-763G A allele and MMP-9 R279Q combinated genes between CI and healthy controls ,and also no statistical significance of distribution of MMP-9 R279Q R allele, Q-type allele and the HL gene combinated genes4.There were significantly difference of HDL(P=0.0482)and hypertension (P<0.001) between cerebral infraction and healthy controls.5. When taking MMP-9 and the HL gene polymorphism locus genotype, age, plasma total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), and drinking history and other potential risk factors into consideration, the Logistic regression analysis showed hypertension (OR=14.277,95%CI=6.574-31.011,P <0.001)was risk factor.,and HDLwas protective factor(OR=0.082<1). MMP-9 geneMMP-9 R279Q (OR = 5.267,95% CI = 2.059-13.471) may be risk factors for CIConclusion:These suggested that in HeBei province Han people MMP-9 R279Q may be related to cerebral infarction , which may be an independent risk factor .MMP-9 C-1562T and HL gene was not the risk factors for CI..There were some relations between combinated genes(C-514T T and R279Q;A-763G G and R279Q) and CI,while no relations between combinated genes(C-514T C and R279Q ;A-763G A and R279Q ;R279Q R and C-514T; R279Q Q and C-514T; R279Q R and A-763G;R279Q Q and A-763G A)and CI.