The Effect Of Oxidative Stress On Erythrocytes Apoptosis In Patients With Chronic Kidney Disease

Objective:To identify the relationship between oxidative stress and erythrocytes apoptosis in chronic kidney disease.Methods:The experimental group: From Mar 2009 to Nov 2009, 97 cases of chronic kidney disease(41 women,56 men, mean age, 48.5±14.8 years, range, 17~81)were recruited. Based on CKD-EPI formula, all patients were divided into 3 groups:stage CKD1~2 ,stage CKD3~4 and stage CKD5 .The patients in stage CKD1~2 were 19 cases of mild mesangial proliferative IgAN(7 women,12men,mean age,46.4±17.8years,range,19~67) diagnosed by renal biopsy and clinical diagnosis. The etiology of patients in stage CKD3~5(34 women,44 men, mean age, 49.0±14.1years, range,17~81) was chronic glomerul-onephritis, hypertensive nephrosclerosis, chronic interstitial nephritis and autosomal dominant polycystic kidney disease.The normal control group consisted of 14 age and sex matched healthy volunteers. Serum malondialdehyde(MDA), superoxide dismutase(SOD) and erythrocytes phosphatidylserine(PS) exposure were measured and clinical data were collected such as hemoglobin, serum creatinie and serum albumin. All these data were undertook statistical analysis and comparison.Results:①Compared with the normal group, hemoglobin concentration, packed cell volume, serum SOD were significantly lower and erythr-ocytes PS exposure, serum MDA were significantly higher(P<0.05) in patients with CKD3~4 and CKD5 stage. Erythrocytes PS exposure and serum MDA in patients with CKD5 stage were two times than the he-althy control group.②The kidney function was increased with Erythrocy-tes PS exposure and serum MDA(P<0.05),and declined with the serumSOD in chronic kidney disease(P<0.05).③The analysis of linear correlatetion showed the percentage of PS positive erythrocytes was negatively correlated with SOD and hemoglobin concentration (r=-0.418and-0.459,P=0.000),positively correlated with MDA(r=0.491,P=0.000);The hemoglobin concentration was negatively correlated with serum MDA(r=-0.410,P=0.000) and positively correlated with SOD(r=0.330,P=0.003).Conclusions: Oxidative stress might contribute to the development of anemia by erythrocytes apoptosis in chronic kidney disease.