| Objective: Clinical investigations have shown that many epileptic patients have deleterious cognitive and behavioral consequences, which have greatly affect the quality of life .To explore the effect of memantine (MEM) on spatial learning and memory, the expression of Calbindin-D28k(Ca-28) in the hippocampus of rats with chronic epilepsy induced by pentylenetetrazol ( PTZ). Methods: Adult male Sprague–Dawley (SD) rats were selected through Y-Maze tests and then divided into 3 groups randomly, normal control (NC)group, PTZ group and MEM + PTZ group. All of their behaviors were observed by Morris water maze. Meanwhile, the expression of mRNA were determined by reverset ranscription polymerase chain reaction (RT-PCR),and the expression of Ca-28 were determined by Western blot respectively. Results: In PTZ group, the learning and memory ability of the epileptic rats was impaired, the expression of mRNA(0.32±0.03 ) and Ca-28 (0.29±0.02) were decreased as compared with NC group mRNA(0.60±0.05 ) and Ca-28 (1.05±0.11) (both P <0. 05). The learning and memory ability of the MEM+PTZ group rats was improved, the expression of mRNA (0.53±0.03)and Ca-28(0.92±0.06) was increased as compared with PTZ group(both P <0. 05). Conclusion: The impairment of learning and memory ability of epileptic rats kindled by PTZ might be related with the decreas of mRNA and Ca-28.MEM can strengthen the expression.
|
PDF Full Text Request