| Objective:The development and progression of colon cancer, which is a complex, multistep progress including mutation or loss of tumour suppressor genes(eg p53) and so on. In recent years, the designated object research which grows regarding the colon cancer tumor already became a main hot topic, particularly it has import significance of diagnosis colon cancer. In clinical work, multivariate analysis has been involved to assess the growth, development and prognosis of colon cancer. Besides tumor size, pathological stage and grade, lymph node status, and ER and PR status, quantitative analysis of some genes on molecular level has been used to evaluate the progression and prognosis. As the only access across the nuclear membrane, nuclear pore complex (NPC) plays an important role in the nucleocytoplasmic shuttling. And nuclear pore proteins (Nucleoporins, Nups), as an important component of NPC, play a "non-transfer" role in the cell signal transduction, and apoptosis. Nup88 was a member of nuclear pore protein complex NPCs family, and was closely related with tumor, and was researched deeply.At present Nup88 as nuclear hole protein complex became the clinical each related professional field research a hot topic alreadly, the high expression in the tumour cell of nuclear hole protein complex Nup88 prompted us:It whether also in colon cancer has high expression rate, whether can grow as the colon cancer tumor designated object.In this research, RT-PCR was used to determine the contants and differences of Nup88 protein and the mRNA expression level in the colon cancer the non-lump mucous membrane, on the remnant end mucous membrane and under the remnant end mucous membrane in order to demonstrate its effect on the development and prognosis of colon cancer. And Nup88 protein were examined by Western blot and immunohistochemical method in the same cancer tissues to investigate its correlation with clinical and pathological features. The relationship between Nup88 and pathological grade, clinical stage, and lymph node status was also studied, Such studies would provided theoretical evidence for the early diagnosis and prognosis assessment of colon cancer.Methods:Malignant and nonneoplastic the Tangshan Workers' hospital pathology department to put on file the wax block paraffin wax organization specimen, the colon cancer organizes has181 examples, nearby the match cancer the non-lump mucous membrane organizes has 84 examples and the shift lymph node organizes has 18 examples. Age 39-88 years old, average 59 years old, position age 63 years old. And masculine 88 examples, feminine 93 examples. Villose+tubular adenocarcinoma has 151 examples, mucilage adenocarcinoma has 30 examples. Splits up has 62 examples, to split up has 88 examples, to split up has 31 examples lowly high..And it Is accompanied by the lymph node shift has 56 examples, non-lymph node shift has 125 examples, Dukes' by stages:A-46 example, B-78 example, C-51 example, D-6 example. Simultaneously collects29 examples which fresh colon cancer organization as well as the cancer the 2cm non-lump mucous membrane organization and cuts the reason mucous membrane nearby in October,2007-2008 year in December.And 20 examples from Tangshan Workers' hospital, 5 examples from the Hebei Medical college first hospital,4 examples from the Hebei Medical college fourth hospital. Age in 37-83 years old, the average age is 61 years old, position age is 60 years old. Masculine has 17 examples, feminine has 12 examples. Villose+tubular adenocarcinoma has 25 examples, mucilage adenocarcinoma has 4 examples. Splition has 4 examples, to splition has 22 examples, to splition has 3 examples lowly high. All diagnoses pass through two high period of service pathology doctor to confirm. The 0.1g pecimens were taken within half an hour from the benign tumor or Colorectal cancer, adjacent non-tumor mucosa, under the stump on the stump mucosa and mucosal tissue, and immediately were frozen by liquid nitrogen and were stored at -80℃refrigerator, to detect the levels of Nup88 mRNA of breast cancer tissue and benign breast tumor tissue by RT-PCR;Western blot was used to detect the contants of Nup88 protein in the Colorectal cancer, adjacent non-tumor mucosa, under the stump on the stump mucosa and mucosal tissue and benign Colorectal cancer, adjacent non-tumor mucosa, under the stump on the stump mucosa and mucosal tissue, to confirm the effect of Nup88 in tissue on the development and prognosis of colon cancer. Collecting the cancer and benign specimens from the same group of patients, to analysis the relationship of Nup88 protein and breast cancer, the relationship between clinicopathological features to explore the Nup88 protein in breast cancer occurrence and development of the role and possible mechanisms. Discusses the Nup88 protein and mRNA expression in the colon cancer occurrence, in the development the possible function mechanism.Results:1,The Nup88 protein in the colon cancer non-lump mucous membrane, the cancer and the shift lymph node's expression colon cancer group and in the lymph node shift cancer group the Nup88 protein's masculine expression rate obviously is higher than nearby the cancer the non-lump mucous membrane group (P>0.05).2,the Nup88 protein's in colon cancer organization expression and the clinical pathology characteristic's relational Nup88 protein's in colon cancer organization expression with the patient sex, the age, the tumor position, the histology type, Dukes'fenqi and the lymph node shift has nothing to do with (P>0.05), with tissue differentiation degree related, splits up that the group its masculine gender expression rate to be higher than obviously lowly splits up the group high (P=0.03).3,the expression of Nup88mRNA in the cancer organizes is highter than nearby the cancer the non-lump mucous membrane, on the remnant end mucous membrane, under the remnant end mucous membrane expression obvious enhancement, the difference has statistics significance (P<0.05); But Nup88mRNA nearby the cancer the non-lump mucous membrane organization (2cm), on the remnant end mucous membrane, under the remnant end mucous membrane's expression, the difference does not have statistics significance (P>0.05).4,Nup88 mRNA and colon cancer clinical pathology characteristic relational: the expression of Nup88 mRNA which in the cancer organizes with the patient sex, the age, the tumor spot, the histology classification, to split up the degree, the infiltration depth, the lymph node shift to have nothing to do with (P>0.05).Concultions:1,The expression of Nup88 is closely related to the growth of malignant tumors, Nup88 involved in the occurrence and development of colon cancer, in the process of cancer cells play an important role.2,Nup88 expression levels are higher in patients with such a high degree of malignancy, poor prognosis and short survival time.3,Nup88 possibly concerns with the colon cancer occurrence.4,the expression of Nup88 mRNA in the cancer organizes with the patient sex, the age, the tumor spot, the histology classification, to split up the degree, the infiltration depth, the lymph node shift to have nothing to do with.In short, Nup88 as an important component of NPC, were significantly increased compared with non-cancerous tissue. There is significant correlation between the expression of Nup88 in colon cancer tissues and colon cancer, histological grade, lymph node metastasis, clinical stage. The resulting high-Nup88 expression and the occurrence of colon cancer is closely related to the occurrence of colon cancer development have a significant impact. and its detection of the expression of Nup88 may assess prognosis of colon cancer more precisely. |
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