The Relevant Research Of The Genotyping And The Clinic Of HBV In Ganzhou Area

This research is based on the collection of 303 Chronic hepatitis B cases whose HBV DNA tested positive of the outpatients and inpatients in the First Affiliated Hospital of GanNan Medical College and GanZhou City's Second People's Hospital. This study uses the Polymerase ChainReaction(PCR)-Reverse Dot Plot for common genotype(A-D type) and detects HBV serum maker,liver function index to confirm the types and distribution frequency of HBVgenotype in GanZhou area. Through the comparative analysis of the HbeAg's positive frequency, HBV DNA's Bacterial load and the comparison of the biochemical index of the liver function part of the different HBV's genotype, we evaluate clinical features of different HBV genotype, which will provide the theoretical basis to treat and prevent HBV in the local area. The main research results list as follows:(1) The HBV genotype results of 303 cases Chronic hepatitis B are:B type 249 patients (82.2%), C type 39 patients (12.9%), B/C mixed type 10 patients (3.3%), D type 3 patienst (1.0%), unclassified 2 patients (0.6%). The main type is B, C type, but obviously the B type is more than the C type (P<0.0001). It also shows that there is B genotype,C genotype, B/C mixed genotype, D genotype, and another two unclassified type in HBV infection in GanZhou area. The main HBV infection is B and C type, particularly, the B genotype is in a dominant position.(2) The relationship between the HBV genotype and HbeAg:the 303 all kinds of hepatitis B patients's HBV DNA tested positive, the total HbeAg positive rate is up to 46.5%, the distribution difference of the HbeAg positive or negative in the B genotype and C genotype has no statistic meaning (P>0.05), but the HbeAg positive rate in C genotype is higher than that of the B genotype.(3) The relationship between the HBV genotype and the HBV DNA's Bacterial load:the load which is more than Ig5 belongs to high load, and there are 103 cases of B genotype belong to the high load (103/249,41.4%).while there are 34 cases of C genotype (34/39,87.2%). The HBV DNA's Bacterial load of C genotype is obviously higher than B genotype's, and the difference has statistic meaning. (4) The relationship between B genotype,C genotype and the damage degree of the liver:through the comparisons of the average of biochemical index, the four biochemical indexes of C genotype except ALB are higher than these of B genotype. (P<0.01或P<0.05).Through the contrast and analysis in article (2),(3),(4), we can conclude that the C genotype's HBV has an active replication, and has a strong pathogenicity, so the infected persons are more likely to be a serious disease of liver.