| Abstract:Objective:To investigate the effects and the mechanisms of two lupus recipes including lupus recipe 1(LR1)and lupus recipe 2(LR2)which was traditional Chinese medicine(TCM) to the MRL/lpr mice.to provide the data and basis for the treatment of systemic lupus erythematosus with traditional Chinese medicine and combined Chinese-Western medicine.Methods:34 MRL/lpr mice were randomly divided into 6 different groups: Group A was LR1.Group B was LR2.Group C was prednisone.Group D was LR1+prednisone.Group E was LR2+ prednisone.Group F was negative control.These were all given liquid every day for total 20 days.Serum anti-dsDNA antibody,Blys and IL-10 were measured by ELISA.The levels of proteinuria in the different groups were measured by Four Bromophenol Blue every 7 days.The changes of kideny morphology were also observed.Results:(1)The change of anti-dsDNA antibody:The level of anti-dsDNA antibody in group A,B,C,D and E were all lower than that of group F (P<0.05).Compared with group A or B,the group D and E were all significant lower (P<0.01). Compared with group C,the group D and E were all lower (P<0.05).(2)The change of Blys:The level of Blys in group A,B,C,D and E were all lower than that of group F (P<0.01).Compared with group A, B or C,the group D and E were all significant lower (P<0.01).(3)The change of IL-10:Compared with the group F,the group A and B were no significant difference(P>0.05) Compared with the group D and E,the group F was significant difference (P<0.01),and group C was no difference(P>0.05).(4)The change of kidney: The level of proteinuria and pathologic damage of kidney in group A,B,C,D and E were all alleviated than that of group F. The LR+ prednisone showed better effect.Conclusion:LR and prednisone should be effective to the treatment of MRL/lpr mice.Both of them can decrease the level of anti-dsDNA antibody,Blys and alleviate the renal pathologic damage.The TCM and glucocorticoid used in combination was superior to that used alone.
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