Influence Of Astragalus Injection On Olfactory Ensheathing Cell Proliferation And Neurotrophic Factors Secretion

Background:Astragalus (astragalus) is a leguminous plant Astragalus for a traditional Chinese medicine Qi.Chinese medicine also believes that astragalus has strengthened the role of the body, clinical re-treatment of hemiplegia Astragalus has good effect of each Shen Yue Jian Application of Astragalus and Chuanxiong Injection treatment of nerve function defect in patients with symptoms were significantly improved; modern pharmacological studies suggest that astragalus howaspects of pharmacological effects, such as immune regulation, anti-bacterial diuretic, cardiac and other role, Shi-Qing Liu et al research of Chinese medicine astragalus on experimental spinal cord injury neuroprotective role that astragalus can increase the body's ability to repair wounds to increase the damageparts of the blood supply is conducive to nerve regeneration.Olfactory ensheathing cells as a special type of glial cells, not only has the promotion of adult mammalian olfactory sensory neurons constantly updated features, but also to promote a variety of the central nervous system neuronal survival and axon growth. Current studies suggest that olfactory ensheathing cells cultured from these functional part of their secretion of a variety of nutrients, such as nerve growth factor, brain-derived neurotrophic factor and so on, olfactory ensheathing cells, the specific biological characteristics that are widely used in clinical,in promoting the recovery of neurological function and improve patient quality of life played a positive role as a new hope for patients with nerve injury.In this experiment the use of integrative medicine in mind, emphasizing the principle of comprehensive treatment, through the mechanism of Chinese medicine treatment study with a view to use in clinical practice of integrative medicine thought to lead to greater neurologic impairment in patients with the Gospel, but also further promote the traditional Chinese medicine to providetheoretical basis.Objective:To observe the effectiveness of astragalus, which promote the proliferation of olfactory ensheathing cells(OECs) and the secretion of neurotrophic factors.Methods:MTT and flow cytometry were used to assess the effects of astragalus in promoting OECs proliferation; enzyme linked immunoassay was used to measure the content of neurotrophic factor.Results:MTT result showed that 20ug/ml and 2ug/ml astragalus could promote OECs proliferation; The result of flow cytometry showed that astragalus could Significantly reduce the number of OECs. Furthemore, the result of ELISA indicated that 2ug/ml astragalus could promote the secretion of neurotrophic factors in OECs significantly.Conclusion:Astragalus can promote OECs proliferation and the secretion of neurotrophic factors.