| Objective:To observe the renal expressions of SDF-1 and CXCR4, and analyze its relationship with renal microvascular abnormalities in the kidney of diabetic rats.Methods:The SD rats were divided into the diabetic group (n=24) and control group (n=24). The diabetic group was treated with a single intraperitoneal injection of streptozotocin (55mg/kg). The expression of SDF-1/CXCR4 in renal tissue were respectively detected by immunohisochemistry method and real-time polymerase chain reaction (real-time PCR) at 8,12,20 and 28 weeks. The relationship between SDF-1/CXCR4 and urinary protein, angiopoietin(Ang-1, Ang-2), endothelial cell markers (CD133, thrombomodulin-1) were analyzed.Results:Compared with control group, the expression of SDF-1 and CXCR4 in diabetic kidney were significantly up-regulated after operation from 8 weeks to 28 weeks with the peak level at 20 weeks (P<0.01), and SDF-1/CXCR4 immunostaining were mainly found in renal tubular interstitium. SDF-1αand CXCR4 mRNA were detected at every time point in control group. The expression of SDF-1αmRNA in diabetic kidney was significantly up-regulated only at 8 and 28 weeks (P<0.05, P<0.01), but difference in expression of CXCR4 mRNA between the diabetic group and the control group was not found (P>0.05). The level of SDF-1 was positively correlated with CXCR4 (r=0.767, P<0.01). The level of SDF-1 or CXCR4 was positively correlated with urinary protein excretion, Ang-2, CD133 and TM-1 in diabetic group, respectively.Conclusion:The abnormal expression of SDF-1/CXCR4 exist in the kidney of diabetic rats, and may be significantly associated with renal microvascular changes. The changes of SDF-1/CXCR4 is partly connected with the development of diabetic nephropathy. |
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