The Investigation Of Umbilical Cord Blood IL-18,IL-5,TNF-α,IL-10 And Their Clinical Value In Early Detection Of Asthma

Objective To measure the levels of IL-18,IL-5,TNF-α,IL-10 in cord blood from neonates of the mothers normal or with asthma and do correlation analysis, evaluate their clinical value in early detection of asthma.Methods Umbilical cord blood was collected from 30 asthmatic lying-in women and 40 healthy lying-in women.Every sample is about 5ml,deal it with a centrifuge, collect the serum and repack it,then storage in-20℃. Serum samples were assayed for IL-18,IL-5,TNF-α,IL-10 using commercial enzyme-linked immunosorbent assay (ELISA).The data was analyzed with SPSS13.0 for t test and Bivariate correlation.it has significant difference when P<0.05.Result 1.IL-18 serum level of neonates with asthmatic mothers (564.17±470.15ng/L) was significantly higher than that of the control group (197.11±96.09ng/L) (P<0.01),so does TNF-αwhose serum level is 118.49±19.98 ng/L (P<0.01).The serum IL-5 is higher in neonates of the mothers with asthma (144.9249±93.53ng/L),but the differences were not statistically significant; IL-10 was significantly decreased in the neonates of asthmatic mothers (20.43±7.86 ng/L),while the control group is 30.505±9.564 ng/L2.TNF-αand IL-10 is correlated, r=-0.409,P=0.025, so does the IL-10 and IL-5,with r=-0.812 and P=0.009.and we also do correlation test between IL-5 and TNF-α, r=0.343,P=0.051,but it was not significant. There was not significant correlation between IL-18 and the other three factors,the related coefficient is-0.102,0.034,0.066,P>0.05.Conclusion 1.Positive family history is an important risk factor for asthma,neonates with this should be given early examine.2.There are significant differences between neonates with asthmatic mothers and their control group of IL-18,TNF-α,IL-10.Although the difference of IL-5 is not significant,the level of IL-5 is higher in asthmatic group. So it has guided meaning of measuring IL-18,IL-5,TNF-α,IL-10 in umbilical cord blood for these neonates who are prone to be affected by asthma.3.we demonstrate that there is correlation among IL-5,TNF-α,IL-10,examine these three factors at the same time will improve the early diagnosis rate.