The Study Of Mutations Of HVRâ…¡ And HVRâ…¢ Of The D-loop Regions Of Mitochondrial DNA In Oral Squamous Cell Carcinoma

ObjectiveThe study is to investigate the mutation of mitochondrial DNA (mtDNA)D-loop hypervariable regionâ…¡(HVRâ…¡) andâ…¢(HVRâ…¢) mutations in oral squamous cell carcinoma (OSCC), to investigate their correlation, and to provide the new targets for the prevention and treatment of OSCC.MethodsThe mtDNA was extracted in the cancer tissues, paracancerous tissues and normal mucosa tissues of 7 OSCC, the D-loop HVRâ…¡and HVRâ…¢regions of mtDNA were amplified by PCR, then were detected by direct sequencing to find the mutantsites after the comparison of all sequencing results with the mtDNA Cambridge sequence in the Gen Bank database, to investigate the relationship between the mutation of mtDNA and OSCC.Results1.82 (56 species) nucleotide changes, with 51 (26 species) nucleotide polymorphism, were found after the comparison of all sequencing results with the mtDNA Cambridge sequence in the Gen Bank database.2.31(30 species) mutations, with 21 located within the HVRâ…¡and HVRâ…¢regions, were found in 3 tumor tissue samples, their paracancerous and normal mucosa tissue were found more polymorphic changes and no mutation; the mtDNA D-loop HVRâ…¡and HVRâ…¢district mutation rate was 42.9%(3/7) in OSCC.3. No mutation was found in all paracancerous tissues and normal mucosa tissues. Conclusion1. The mtDNA D-loop HVRâ…¡and HVRâ…¢regions are highly polymorphic regions and reflected a high degree of mtDNA mutation.2. The D-loop HVRâ…¡and HVRâ…¢regions of mtDNA might play a significant role in the tumorigenesis of OSCC.3. It may become new targets for the gene therapy of OSCC by regulating the above indexes.