MiR-210 And Target MRNA Expression In The Kidney After Ischemic-reperfusion Injury (IRI)

Renal ischemia injury is in relation with renal transplantation and renal operation. As an important pathological process, the timely reconstruction of renal blood flow in ischemic region is a key treatment for renal ischemic injury. Animal experiments and clinical studies showed that compensatory angiogenesis was increased after issues ischemia. Most studies have confirmed that the VEGF pathway signalling has the critical regulatory role in endothelial cell proliferation, differentiation, migration and angiogenesis and involved in the regulation of ischemia-induced angiogenesis in tissue ischemia, which are perhaps the most important mechanism in regulation of angiogenesis in renal ischemia. Recently, studies have showed that microRNAs (miRNAs) are small noncoding RNAs and regulate gene expression at the post-transcriptional level by either degradation or translational repression of a target mRNA that regulate physiological and pathological processes. And miRNAs targeting endothelial cell function and angiogenesis in tissue ischemia-induced have been identified. Our results showed that miR-210expression changes after renal ischemia may be involved in targeting Ephrin-A3 to regulate angiogenesis.MethodsMice were subjected to a standard renal I/R to induce acute kidney injury (AKI) after 40 min of bilateral renal artery clamping. miR-210 expression changes used Quantitative Real-time RT-PCR analysis at 4h following I/R. Ephrin-A3 mRNA expression changes used Quantitative Real-time RT-PCR analysis at 4h ,24h,48h,72h,7d following I/R. Ephrin-A3 protein expression changes used immunochemistry at 4h,24h,48h,72h,7d following I/R.Results1. Ephrin-A3mRNA expression were increased in I/R injury at 4h compared to the sham controls ( P< 0.01).2. Ephrin-A3 protein expression was decreased in I/R injury at 4h compared to the sham controls ( P< 0.01).3. Quantitative Real-time RT-PCR analysis showed that ischemic kidney injury significantly increased miRNA-210 expression compared to the sham controls, with prominent changes at 4h after recovery.( P< 0.05).ConclusionRenal I/R to induce acute kidney injury (AKI) significantly increased miRNA-210,expression with prominent changes at 4h after recovery., Ephrin-A3 mRNA expression were increased in I/R injury compared to the sham controls. And Ephrin-A3 protein expression were decreased in renal I/R injury compared to the sham controls .These results provided evidence that miR-210 may be somehow involved in the mechanisms of renal ischemic injury disease through targeting Ephrin-A3 to regulate angiogenesis.