| Objective:1. To investigate the expression of epidermal growth factor receptor (EGFR) and interrelation with some factors in the specimens.2. To analyze the incidence and profiles of EGFR mutation in cervical cancer, which provide gene detection methods and theoretical basis for the targeted treatment of cervical cancer.3. To investigate the relationship between the expression of EGFR and the immutation of EGFR.Methods:1. Paraffin-embedded tumor tissue samples were obtained from 77 patients with cervical cancer. The EGFR protein expression were detected by immunohisto-chemical (IHC) method.2. Paraffin-embedded tumor tissue samples were obtained from 86 patients with cervical cancer. The deletion mutation in EGFR exon 19 were detected by PCR and the point mutation in EGFR exon 21 was detected by PCR-RLFP. The positive mutation of EGFR gene were detected by DNA sequencing.Results:1. The positive expression rate of EGFR was zero in normal cervix,42.9%(6/14) in preinvasive cervical carcinoma,76.7%(33/43) in invasive cervical cancer. The expression rate of EGFR protein was significantly correlated among the three groups and between each of them, with the progress of clinical stage, EGFR positive expression tatio increased (P<0.01). In invasive cervical cancer, The expression rate of EGFR protein was significantly correlated with the clinical stage(P<0.01), but had no relation with histological grade,age or pathological type(P>0.05).2. No EGFR gene mutation were detected in exons 19 and 21 of cervical cancer samples by PCR, PCR-RLFP and DNA sequencing method.Conclusions: 1. There was an over-expression of EGFR in cervical cancer.The rate of EGFR protein expression related to the clinical stage, but had no relation to the age, histological grade or hitologic type.2. There was no EGFR mutation in exon 19 and 21 in cervical cancer.3. The abnormal expression of EGFR protein had no relation to EGFR mutations, while maybe relate to other EGFR mutations in cervical cancer. |
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