The Effect Of I.v. Injection Of Terbutaline Early On Rats With Acute Lung Injury

Objective To observe the effect of intravenous injection of terbutaline early on rats with acute lung injury.Metheds 40 SD rats were randomly assigned into 4 groups:control group(group A), ALI group(group B), ALI+terbutaline treatment group(group C) and rats with acute lung injury, NS ALI+terbutaline+ouabain intervention group (group D),10 in every group. The rats of every group were respectively anesthetized by intraperitoneal injection of 10% Chloral Hydrate (0.35 ml/100 g). After being anaesthetized successfully, the rats in group A were injected 1 ml/kg NS by the tails vein within 1 hour; the rats in group B were injected 5 mg/kg LPS (which dissolved in 1 ml/5 mg of NS) by the tails vein in 4 times within 1 hour; the rats in group C and D were injected 5 mg/kg LPS (which dissolved in 1 ml/5 mg of NS) by the tails vein in 4 times within 1 hour, after then, the rats were injected 0.5 ml/100 g terbutaline sulfate injection (10-3 mol/L) by micro-pump within 1 hour. Then elevated the rats'head to 45 degrees, the tracheal intubation was performed under lights illuminating the rats'neck, after intubated successfully,1 ml/kg Ringer's solution was dripped into trachea in group A, B and C, and the group D with 1 ml/kg Ringer's solution which contain 10-3 mol/L ouabain. After 6 hours of establishing the model of acute lung injury in rats, pathomorphologic changes were observed, the pathomorphologic scores of lung injury were calculated, and the wet to dry weight ratio (W/D), and the activity of Na+-K+-ATPase, and bronchoalveolar lavage fluid (BALF) cells count, the percentage neutrophilicgranulocyte (PMN), BALF concentration of protein were determined.Results The pathomorphologic scores of lung injury, the wet to dry weight ratio (W/D), bronchoalveolar lavage fluid (BALF) cells count, the percentage neutrophilicgranulocyte (PMN), BALF concentration of protein in group A (2.6±0.8,3.92±0.46,4.08±0.76×108,16.11±3.63%,0.17±0.07 g/L) were significantly lower than that in group B, C, D (12.2±3.2,5.12±0.48,10.67±1.84×108,47.2±6.03%,0.39±0.07 g/L; 8.4±1.3,4.49±0.34,8.84±1.43×108, 34.55±7.34%,0.28±0.06 g/L; 11.3±1.7,4.98±0.31,10.67±1.61×108,44.93±7.51%,0.37±0.06 g/L; all P<0.05), the above mentioned parameters in group B and D were higher than those in group C(all P<0.05), but there were no significant differences between group B and D(P>0.05), the activity of Na+-K+-ATPase in group A (18.188±2.081 U/mgprot)were significantly lower than that in group B, C, D (11.235±1.730 U/mgprot; 14.211±1.658 U/mgprot; 11.400±1.798 U/mgprot; all P<0.05), the activity of Na+-K+-ATPase in group B and D were higher than those in group C (all P<0.05), but there were no significant differences between group B and D (P>0.05).Conclusion The intravenous injection of terbutaline early can promote alveolar epithelial fluid reabsorption in rats with ALI via the upregulation of the activity of Na+-K+-ATPase in lung,and the effect can control the further development of ALI.