| Objective:To fill in method of nourishing yin, filling pith and benifiting brain of focal cerebral ischemia in rats behavior and brain morphology and neural biochemical markers of experimental research, reveals Ziyin tiansui Yinao France on cerebral missing Blood-The role of reperfusion and its mechanism.Method:1. Choice of male Sprague-Dawley rats, SPF grade, weighing 250g-280g, according to the principle of randomization, were divided into five groups, Were sham-operated group, model group, nourishing yin, filling pith and benifiting brain side group, Buyang Huanwu Tang group, nimodipine group, n=15. Sham-operated group to do separate artery surgery, not the middle cerebral artery embolization. Model group, nourishing yin,filling pith and benifiting brain side group, Buyang Huanwu Tang group, nimodipine group underwent middle cerebral artery embolization. nourishing yin, filling pith and benifiting brain side Buyang Huanwu Tang, nimodipine and 70kg respectively, according to the daily adult 121g, 80g,90mg dose calculation, Middle cerebral artery occlusion 24 hours after starting the corresponding group of drugs fed continuously fed 7 days, once a day, Dose, respectively nourishing yin, filling pith and benifiting brain side 10.8g/kg, Buyang Huanwu Tang 7.15g/kg, nimodipine 8.0mg/kg (calculations are based on the amount of crude drug), sham operation group and model group were with normal saline 10ml/kg gavage.30min after the last dose were decapitated brain.2. The results of observation,1) infarct volume determination; 2) The histopathological observation; 3) serum T-SOD, and cu-zn-SOD determination; 4) The serum levels of malondialdehyde (MDA); 5) Bcl-2, Bax immunohistochemical staining; 6) The rieuronal apoptosis detection (TUNEL method).Results:1:Display nourishing yin, filling pith and benifiting brain side Buyang Huanwu Tang, nimodipine on average, reduce infarct size effect, However, the effect of nimodipine was weaker than nourishing yin, filling pith and benifiting brain and Buyang Huanwu Tang.2:The brain tissue pathological examination: nourishing yin, filling pith and benifiting brain side Buyang Huanwu Tang group was significantly better than the model group; 3:nourishing yin, filling pith and benifiting brain side group was significantly increased T-SOD activity and lower MDA content in; 4:The right brain tissue injury in Bcl-2, Bax expression: nourishing yin, filling pith and benifiting brain side group, Buyang Huanwu Tang group, nimodipine significantly increased bcl-2 expression, Bax expression decreased, Bax/bcl-2 significantly lower than ischemia-reperfusion group (P all "0.01). Which nourishing yin,filling pith and benifiting brain side group decreased compared with Bax/bcl-2 Buyang Huanwu Tang group, nimodipine group even more pronounced (p<0.01).5:Compared with model group, fill the marrow Yinao side group, Buyang Huanwu Tang group, nimodipine rat ischemic brain tissue of the number of apoptotic neurons significantly reduced (P<0.01), among the three groups wither The number of dead cells, no significant difference (P> 0.05)Conclusion:Through the experimental findings suggest that marrow nourishing yin,filling pith and benifiting brain side to effectively reduce the ischemia-reperfusion of the infarct area after, to improve the infarcted region of brain tissue repair, method of nourishing yin, filling pith and benifiting brain to raise the animal model of serum SOD, Cu-Zn-SOD activity in, indicating that benefits nourishing yin fill in myeloid cells in the brain side of the brain tissue lipid peroxidation damage in a significant protective effect, nourishing yin fill in the brain may benefit significantly promote myeloid ischemic cortical neurons the expression of Bcl-2 also inhibit the expression of the role of Bax, that have reduced the role of Bax/Bcl-2, So you can speculate method of nourishing yin, filling pith and benifiting brain could inhibit neuronal apoptosis and thus play a neuroprotective role, while the anti-apoptotic effect may be achieved through lower Bax/Bcl-2.
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