| 1 Using the SD rats model which established as severe pancreatitis, and gave Wu-yao, Da-huang decoction. After that the structural damage and repair of tissue would be observed in the optical microscope, which is pancreas, colon, small intestine, pancreas, small intestine ratings. It will be demonstrated from perspective of pathology that Wu-yao and Da-huang de-coction can protect the intestinal mucosal barrier of severe acute pancreatitis, and also reason-able for therapeutic alliance. It can provide the useful methods for protecting the intestinal mucosal barrier of clinical severe acute pancreatitis.2 We observed the release of abdominal distension, abdominal (bladder pressure measure-ment), ACAPHE-Ⅱscore, Testing of indicators of neutrophils (N), C-reactive protein (CRP), D-lactate, diamine oxidase (DAO), serum endotoxin in severe pancreatitis patients to invest-igate the protection of Wu-yao and Da-huang decoction in intestinal mucosal barrier of severe acute pancreatitis. And further evaluate the protective effects of therapeutic alliance of Wu-ya o and Da-huang to the intestinal mucosal barrier of severe acute pancreatitis.150 SD rats were established severe acute pancreatitis model was randomly divided into four groups, the control group(groups A)I fed 0.9% sodium chloride injection, after modeling the death or killed in the fourth days get pancreas, small intestine and the colon to send patho-logical examination,Da-huang group (groups B) I fed Da-huang decoction gavage, Wu-yao group (groups C) I fed Wu-yao decoction gavage, Wu-yao, Da-huang group (groups D) I fed Wu-yao, Da-huang decoction gavage, intervene in 3 days (the 4 day), all were killed, pancre-as, small intestine and the colon to send pathological examination.2 Select 27 cases meet the clinical inclusion criteria in patients with severe acute pancreas-titis were randomly divided into three groups, the control group (groups A), Da-huang group (groups B), Wu-yao, Da-huang group (groups C). After Confirm the diagnosis were treated with basic therapy (Fluid resuscitation, inhibit pancreatic secretion, anti-infection, etc.) and Three groups gavage and enema. Group A,50% magnesium sulfate gavage,or magnesium sul- fate and glycerin enema; GroupB received Da-huang decoction high retention enema; GroupC was given Wu-yao decoction gavage and Da-huang decoction high retention enema;Measured daily in all patients abdomen, abdominal pressure, neutrophils, C-reactive protein, ACAPHE-Ⅱscore, and collect admission and peripheral blood 1,3,5,7…days to detect D-lactate,diam-ine oxidase, endotoxin.1 GroupA,GroupB,GroupC,GroupD of glands,mucous membrane changes in the structu-re,repair and ratings,There was significant difference. A group of complete necrosis of pancr-eatic tissue, acinar knot enough to disappear; B group of structural elements of the pancreas damage, inflammatory cell infiltration, interstitial edema, vascular dilatation and congestion, some acinar clear structure; C group structural damage pancreas, lymphocytes and neutrophils cell infiltration, interstitial edema, vascular dilatation and congestion of a small amount of res-idual pancreatic gland; D group to varying degrees of pancreatic hemorrhage, vascular conge-stion, inflammatory cell infiltration, local peripancreatic fatty tissue necrosis; A group of sev-ere intestinal damage, intestinal submucosa, muscularis and serosa layer of necrosis, hair loss, inflammatory cell infiltration; B group of small intestinal damage, villous necrosis, inflamma-tory cell infiltration, edema, remnants of a small amount of glandular lamina propria; C group a large number of intestinal inflammatory cell infiltration, dilatation and congestion of villous blood vessels, occasional epithelial cell necrosis, shedding; D group forms the basic integrity of the small intestine villi, some villus blunting, lamina propria glands is rich in a small amou-nt of interstitial inflammatory cell infiltration; A group of intestinal injury severity, mucosal 1-ayer, muscularis and serosa layer of necrosis; B group of colorectal mucosa can be seen more inflammatory cell infiltration, some glandular destruction, mucosal and submucosal vascular dilatation and congestion; C group of colorectal mucosa shows more inflammation cell infiltr-ation, some glandular destruction, mild vascular dilatation and congestion; D group of colore-ctal mucosa integrity, and gland structure intact, a small amount of interstitial inflammatory c-ell infiltration.2 Three groups in the clinical indicators of abdominal circumference, abdominal pressure, APACHE-Ⅱscore, Neutrophils significant difference (P<0.05), whereas C-reactive protein was no significant difference; There are significant differences in three groups for D-lactate, diamine oxidase (DAO), serum endotoxin assay (P<0.05), and the number of days between each group (P<0.05).1 Da-huang and Wu-yao decoction can repair tissue injury of colorectal mucosa of severe acute pancreatitis. Da-huang decoction also indue the diarrhea. It would be better for therap-eutic alliance of Wu-yao and Da-huang to the intestinal mucosal barrier of severe acute pancr-eastitis. 2 Wu-yao and Da-huang synergistical protect the intestinal barrier dysfunction of severe acute pancreatitis. |
