Expression Of Chemokine Receptor CCR7 And Chemonkine SLC, ELC And Its Association With Lymph Node Metastasis In Breast Carcinoma

Abstract:. Objective:To detect the expression o f chemokine receptor CCR7 and chemokine SLC,ELC protein in breast carcinoma and discuss their relationship with lymph node metastasis. Method:1.The expression of chemokine receptor CCR7 and chemokine SLC,ELC protein in 48 cases breast carcinoma,23 cases metastatic lymph node,15 cases normal tissue beside carcinoma,15 cases fibroadenoma were detected by immunohistochemicaltechnique.2. The chemotaxis and invasion assays were performed on breast cancer cells-MCF-7 in presence of exogenous SLC and ELC;Detect the expression of SLC and ELC in novel lymph node with breast cancer metastasis by Westen-Blot.Second replace the exogenous SLC and ELC with the clear supernatant liquid of novel lymph node with breast cancer metastasis to study the chemotaxis and invasion of breast cancer cells-MCF-7 facilitated by endogenous SLC and ELC,and when SLC and ELC was blocked Result:â‘ There were 30 cases of positive expression of CCR7 protein in 48 cases of breast carcinoma tissue62.5%(30/48),20 cases in 23 cases of metastatic lymph node 87.0%(20/23),5 cases in 15 cases of normal tissue beside carcinoma33.3%(5/15),3 cases in 15 cases of fibroadenoma20.0%(3/15). The positive expression of CCR7 protein in metastatic lymph node was significant than that in other teams.P<0.05. The positive expression of CCR7 protein in breast carcinoma with lymph node metastasis was significant than that without lymph node metastas.P<0.01. The positive rate in clinical stageâ… â…¡,â…¢has not significant difference P>0.05.The positive expression of CCR7 was correlated with lymph node.â‘¡There were 32 cases of positive expression of SLC protein in 48 cases of breast carcinoma tissue66.7%(32/48),21cases in 23 cases of metastatic lymph node91.3%(21/23),7 cases in 15 cases of normal tissue beside carcinoma46.7%(7/15),6 cases in 15 cases of fibroadenoma40.0%(6/15). The positive expression of SLC protein in metastatic lymph node was significant than that in other teams.P<0.025. There was not significant difference between carcinoma, normal tissue beside carcinoma and fibroadenoma.The positive expression of SLC protein in breast carcinoma with lymph node metastasis was significant than that without lymph node metastas.P<0.05. The positive rate in clinical stageâ… â…¡,â…¢has significant difference P<0.05.The positive expression of SLC was correlated with lymph node and promote invasion.â‘¢The expression of ELC has similary result with SLC protein.â‘£Both exotgenous and endogenous SLC and ELC can facilitate the chemotaxis and invasion of the breast cancer cells-MCF-7,both chemotaxis and invasion could be blocked by neutralazing anti-SLC,ELC antibody. Conclusion:CCR7/ SLC,ELC axis may contribute to lymph node metastasis and promote invasion in breast cancer.