The Protective Effect Of Polygonum Preconditioning On Renal Ischemia-reperfusion Injury In Rats

Objective:To investigate the protective effect and mechanisms of polygonum on renal ischemia-reperfusion injury in rats. Methods:72 SD male rats were randomly divided into four groups:sham-operation group, ischemia-reperfusion group, polygonum cuspidatum preconditioning group and polydatin (PD) preconditioning group.Every group was divided into three groups in different time points(Before ischemia 10min, after ischemia-reperfusion 6,12 h).Six rats were conducted to experiment in each time point. The rat's RIRI model was established by resecting the right renal firstly, followed by blocking the blood flow of left renal for 60 min. Renal function was assessed by measuring serum creatinine and blood urea nitrogen.The MDA content were measured by Thibabituric Acid technique and SOD activity were measured xanthine oxidase technique. The protein expression of NF-κB, ICAM-1 and ET was measured immunohistochemistry. ELISA assay of renal tissue levels of TNF-a, and were compared. Results:1. The model group were found epithelial cells edema of renal tubule, vacuole-zation,tuble dilation, shrinkage of basement membranes,nuclear fragmentation and various tuble casta,while all these histopathological changes were relieved in treatment group。2. I/R group,polygonum cuspidatum group and PDgroup showed significant increase in Scr,BUN,MDA,TNF-,the expression of NF-κB,ICAM-1,ET(P<0.05)and decrease in SOD compared with sham-operation group at 6,12 hours after renal reperfusion. All preoperativelOmin groups have no difference with ham-operation group.3. Polygonum cuspidatum preconditioning group and PD preconditioning group showed significant decrease in Scr,BUN,MDA,TNF-α,the expression of NF-κB,ICAM-1 compared with the I/R group at 6,12 hours after renal reperfusion (P<0.05).4. Polygonum cuspidatum preconditioning group and PD preconditioning group showed increase in SOD compared with I/R group at 12 hours after renal reperfusion(P<0.05). PD preconditioning group have no difference with the ischemia-reperfusion group at 6 hours after renal reperfusion.5. Polygonum cuspidatum preconditioning group showed significant decrease in the expression of ET compared with the I/R group at 6,12 hours after renal reperfusion(P<0.05). PD preconditioning group have no difference with the I/R group.6. Polygonum cuspidatum preconditioning group and PD preconditioning group showed significant decrease in TNF-a compared with I/R group at 6 hours after renal reperfusion(P<0.05),and have no difference with each other at 6 hours after renal reperfusion. Conclusions:In the process of renal I/R injury polygonum cuspidatum can enhance SOD activity, reduced MDA, ICAM-1, NF-κBp65, ET, to improve renal function, which played a major role in PD but there are other ingredients play a role.