Polymorphism Of COMT,P21 And NuMA In Sporadic Breast Cancer

Objective:To explore the relationship between the single nucleotide polymorphism of Catechol-O-methyltransferase(COMT) gene,Cyclin-dependent kinase inhibitor (P21) gene and Nuclear mitotic apparatus protein (NuMA) gene with the development of sporadic breast cancer in female population in Shandong Province. Methods:A Val158Met polymorphism in COMT gene,a Ser31 Arg polymorphism in P21 gene and a Ala794Gly polymorphism in NuMA gene were analyzed using allele specific amplification (ASA) in 140 cases with sporadic breast cancer and 122 samples of normal controls. The difference of genotypic distribution frequency between the groups was analyzed. Results:The frequency of COMT Met/Met genotype in case group and control was 27.1%(38/140) and 8.2%(10/122), respectively. The frequency of allele containing genotypes is significantly higher in breast cancer patients than in normal controls (χ2=15.638,P<0.001). The proportion of variant genotype increased as clinical stage (OR=5.00, P<0.001) and histological grades(OR=5.40, P<0.001) advanced, as well as with increased numbers of lymph node metastasis(OR=3.00, P=0.002). The frequency of P21WAF1 Ser/Arg genotype in case group and control was 54.3%(76/140) and 42.6% (52/122), which showed no significant difference(χ2=3.549,P=0.060). The frequency of NuMA Ala/Gly genotype in case group and control was 15.7%(22/140) and 16.4% (20/122), which showed no significant difference(χ2=0.020,F=0.886). Conclusions: These data suggest that COMT Val158Met variants may play a role in the development of sporadic breast cancer in female population in Shandong Province, which can be a diadynamic criteria for early breast cancer.