| Objective:To study the effects of picrosideⅡon cerebral ischemic reperfusion injury in rats.Methods:Intraluminal thread methods were applied to establish the middle cerebral artery occlusion reperfusion models in rat. PicrosideⅡ(10mg/kg-1) and Salvianic acid A sodium(10mg/kg-1) were injected from the tail vein for treatment. The nervous behavioral function was evaluated with Bederson's test. The cerebral infarction volume was observed with tetrazolium chloride(TTC) staining. The structure of cells were oberserved with Hematoxylin-Eosin. The structure of nissy body of cells were determined by toluidin blue. The expressions of (toll-like receptor4, TLR4), (nuclear kappaB, NFκB) and (inhibitor kappaB, IκB) were determined by immunohistochemical assay. The concentration of NFκB and IκB in brain tissue was determined by ELISA. TUNEL positive cells were counted by immunofluoresence assay. Results:The nevous behavioral malfunction was normal and there was no focus infarction in sham operative group. The structure of cells and the structure of the nissy body were normal. The expression of TLR4,NFκB and IκB were weakly and the apoptotic cells were scattering in brain tissue in the sham operative group. In the negative control group, the nevous behavioral malfunction and focus infarction was higher than those in sham operative group. Damaged neurons exhibit features including pyknosis, karyorhexis and shrunken cell bodies in the negative control. In the negative control group, the number of TUNEL positive cells and the expression of TLR4,NFκB and IκB increased, the concentration were significantly higher than those in the shame operative group(P<0.05). While in the positive control and picrosideⅡgroups, the nevous behavioral malfunction, the focus infarction, the expressions and concentration of TLR4,NFκB,IκB and the the number of TUNEL positive cells were significantly lower than those in the negative control group (P<0.05). There was no significant difference between the positive control group and PicrosideⅡgroup (P>0.05). Conclusion:PicrosideⅡmight downregulate the expression of TLR4,NFκB and IκB to inhibit neuronal apoptosis induced by inflammation after cerebral ischemia reperfusion injury in rats.
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