| Ischemic brain stroke is one of the frequently occurred neurological diseases with high morbidity rate. In modern society, the increasing incidence of it has brought a heavy burden to society and family. Modern medical treatment of cerebral ischemia mainly focused on revascularization and neuroprotection which both stress the rescue of peripheral tissues around the ischemic core during acute phase of cerebral ischemia. However, there still are a considerable number of cerebral ischemic patients left with varying degrees of neurological impairment. Therefore, the neurological rehabilitation is particularly important to patients in the late phase after cerebral ischemia. Angiogenesis around ischemic area can restore its oxygen and nutrient supply and is beneficial to the newly generated neurons, which can promote nerve regeneration and functional remodeling in brain, key to rehabilitation of the neurological function. Resveratrol is a chemical compound extracted from plants with wide biological effects. In recent years some research suggested that resveratrol can promote angiogenesis after myocardial ischemia. In this study we used mice cerebral ischemia/reperfusion model to investigate the effects of resveratrol in promoting angiogenesis and its relationship with the angiogenesis relevant factors. The aim of this study is to discuss mechanisms of protective effect of resveratrol on cerebral ischemia reperfusion, which will provide a theoretical basis for clinical application.Objective To confirm resveratrol's promotion effect to angiogenesis and rehabilitation of neurological function in mice after cerebral ischemia reperfusion, and further research its relevance to the expression of angiogenesis associated factors. Methods Male BALB / c mice were randomly divided into ischemia control group (IG) and resveratrol-treated group.(RG). Mice in RG treated with intragastric gavage of resveratrol while mice in IG treated with saline gavage as control. The surgery of middle cerebral artery occlusion (MCAO) was operated on the mice in both groups with sham control. One Day, the third day, 5th day, 7th day, and 14th day after reperfusion evaluation of neurological function grades was carried out on mice respectively and brains were removed from euthanized mice with overdose anesthesia. Next, the brains tissue was fixed then embedded or protein was extracted. Immunohistochemistry was employed to determinate Microvessel density, and Western blot was performed to analyze the expression of angiogenesis associated factors. Neurological score of mice in RG is lower than in IG at each time point (P <0.05), and decreased much rapidly through time. The MVD count in RG is statistically different from which in IG (P <0. 05). The expression of VEGF in RG were always higher compared with that in IG (P <0.05),and showed a significant increase in early phase(the 3rd day),reaching a peak on 7 day, while the expression of VEGF in IG showed a trend of slow increase. The expression of other angiogenesis associated factors such as Basic fibroblast growth factor (bFGF), platelet derived growth factorβ(PDGF-β), and insulin-like growth factor (IGF-1) were similar to VEGF expression. Moreover, the expression of angiogenesis associated factors in RG were statistically different from the expression in IG 5 days after ischemia and reperfusion (P <0.05). Conclusion Resveratrol can promote the progress of angiogenesis and neurological function rehabilitation after ischemia-reperfusion in mice, which may benefit from the induction of angiogenesis related factors'expression during the early phase after cerebral ischemia.
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