Research On A Collagen Ⅰ-sodium Hyaluronate-fibrin Glue Tri-copolymer Scaffold Bonding With Antigen-extracted Bovine Cancellous Bone

The articular cartilage damage is a common clinical disease, which most happens in the knee and hip joints for there greatest tolerance. However, the self-repair capacity of cartilage is limited, and the articular cartilage damage often associated with subchondral bone damage. Meanwhile,the pathological changes of long bone metaphyseal will lead to the destruction of cartilage and bone defects, no matter where it happens.There are many treatment for the osteochondral defects, and tissue engineering is a hot research. Our study designed and constructed a new type of osteochondral scaffold and explored the following study. The results show that the scaffold material can meet adhesion, growth and proliferation of the cell , which also have a good result to the repair of rabbit articular cartilage defect.1. Preparation and structural characteristics of collagenⅠ-sodium hyaluronate-fibrin glue tri-copolymer scaffold bonding with antigen- extracted bovine cancellous bone Objective:To explore the preparation and structural characterization of collagenⅠ-sodium hyaluronate-fibrin glue tri-copolymer bonding with antigen-extracted bovine cancellous bone. Methods:Prepare the rat tail collagen and antigen-extracted bovine cancellous bone by ourselves. The osteochondral scaffold was constructed of collagenⅠ-sodium hyaluronate-fibrin glue tri-copolymer scaffold and antigen-extracted bovine cancellous bone with adhesion of the two parts by fibrin glue.Then it's freeze-dried cross-linked by carbodiimide for 24h. Pore size and internal pores of the osteochondral scaffold were observed by gross and SEM. Results:The osteochondral scaffold after crosslinking and adhesion had the porous structure. The bone scaffold was bonding with the cartilage scaffold solidly. Pore size of bone scaffold is about 300 ~500μm and pore size of cartilage is about 80~120μm. Conclusion:The osteochondral scaffold of the collagenⅠ-sodium hyaluronate-fibrin glue tri-copolymer scaffold bonding with antigen-extracted bovine cancellous bone has normal morphology .It can meet the structural requirements of the cell adhesion, growth and repair defects in vivo.2. The research of the biocompatibility of collagenⅠ-sodium hyaluronate- fibrin glue tri-copolymer scaffold bonding with antigen-extracted bovine cancellous bone.Objective : To explore the biocompatibility of collagenⅠ-sodium hyaluronate-fibrin glue tri-copolymer scaffold bonding with antigen-extracted bovine cancellous bone. Methods:Rabbit bone marrow stromal cells were isolated and amplified before they were seeded into the scaffold at the third passage. The proliferation of the cells on the scaffolds was examined using MTT method and the growth curve was drawn. The scaffolds with cells were dehydrated and observed by SEM after 1d and 7d. Results:The examination under SEM indicated that the BMSCs adhered and spread well on the scaffold after 1d and 7d, and the extracellular matrices were also observed around the BMSCs after 7d. Conclusion : The osteochondral scaffold of the collagenⅠ-sodium hyaluronate-fibrin glue tri-copolymer scaffold bonding with antigen-extracted bovine cancellous bone has a good biocompatibility , which make it a useful scaffold in the osteochondral tissue engineering.3. The research of the repairing osteochondral defects in rabbits by collagenⅠ-sodium hyaluronate-fibrin glue tri-copolymer scaffold bonding with antigen-extracted bovine cancellous bone.Objective:To explore the repair of collagenⅠ-sodium hyaluronate-fibrin glue tri-copolymer scaffold bonding with antigen-extracted bovine cancellous bone in vivo. Methods:12 New Zealand Rabbit were used as experiment animals. After making out defects 5mm in diameter, six knees of the rabbits were filling up with nothing in random, and others were filling up with collagenⅠ-sodium hyaluronate-fibrin glue tri-copolymer scaffold bonding with antigen-extracted bovine cancellous bone. After 12 weeks and 24 weeks all animals were killed ,then the newly tissues were observed by gross obversation and histology. Results:After 12 weeks and 24 weeks, tissue defects of the experimental group can be filled with the scaffolds and has the smooth surface, while the control group in the articular surface shows irregular structures. The scaffolds after 12 weeks were absorbed and the defects were repaired with hyaline cartilage. After 24 weeks, the new issues were similar to normal cartilage and the cancellous bone has basically been degraded. Conclusion:collagenⅠ-sodium hyaluronate-fibrin glue tri-copolymer scaffold bonding with antigen- extracted bovine cancellous bone has a good restoration for the osteochondral defects of rabbits and can be expected to be an osteochondral tissue engineering scaffold.