| Chemoresistance is the main obstacle which restricts the effect of chemotherapy on cancer cells. Why cancer cells produce chemoresistance and how to solve this problem is an urgent concern for oncologists. The major factor or dominant mechanism underling chemoresistance is still unknown, though some mechanisms have been reported, such as the increase of drug effluence, the strengthening of non-specific detoxification process, the enhanced DNA damage repair and apoptosis inhibition. Recent study has shown that the Nrf2-Keapl-ARE signaling pathway may be the key chain linking a variety of mechanisms for chemoresistance, and cancer cells may regain chemosensitivity through genetic engineering on this pathway. We observed the different Nrf2 expressions in A2780cp, A2780, SKOV3 and Hey cell lines as well as the change of their chemosensitivity through Nrf2 siRNA transfection. The result may provide a theoretical basis for the treatment of ovarian cancer.1.The cisplatin chemoresistance of several ovarian cancer cells and the Nrf2 protein expression in these cellsObjective:To detect the Nrf2 protein expression in A2780cp,A2780,SKOV3 and Hey cell and explore the effects of cisplatin on A2780cp, A2780, SKOV3 and Hey cell lines.Methods:Ovarain cancer cell line A2780cp, A2780, SKOV3 and Hey were resuscitated, incubated and divided into 2 groups:one included A2780cp and A2780 cell lines, another included SKOV3 and Hey cell lines.SRB was used to investigate cisplatin effects on their growth. The concentration of cisplatin was 1μg/ml,2μg/ml,4μg/ml,8μg/ml.The samples were observed for 72hr respectively. Western-blot was used to investigate Nrf2 protein expression in these cells. SPSS 16.0 software was used to analysis the data by single factor variance method. Results:The effect of the cisplatin inhibiting to this four cell lines proliferation is related with the concentration. There are difference in inhibition between the two cell lines in each group(p<0.05).So the A2780cp and SKOV3 cell lines are realatively chemoresistance to cisplatin while A2780 and Hey cell lines are relatively sensitive. The Nrf2 protein expression is quite different in each two cell lines (p<0.05). The Nrf2 protein is highly expressed in A2780cp and SKOV3 cells and extremely low expressed in A2780 and Hey cells.2. The effect to the ovarian cancer cells of siRNA transfection in the Nrf2 protein expression and the cisplatin sensitivityObjective:To observe the change of the Nrf2 protein expression in A2780cp and A2780 cell lines after Nrf2 siRNA transfection.Explore the effects of cisplatin on A2780cp and A2780 cell lines both before and after trasfection. Methods:Ovarain cancer cell line A2780cp and A2780 were incubated and be trasfected before western-blot was used to investigate Nrf2 protein expression in the treated cells. SRB was used to investigate the difference in cisplatin effects. The concentration of cisplatin was:A2780cp and A2780cp_kd:4μg/ml,8μg/ml,16μg/ml,32μg/ml.A2780 and A2780_kd:1μg/ml,2μg/ml, 4μg/ml,8μg/ml. FCA was used to detect the A2780cp and A2780cp_kd cells apoptosis rate. SPSS 16.0 software was used to analysis the data by single factor variance method. Results: The Nrf2 protein is low expressed in A2780cp_kd and A2780 cell lines. The chemoresistence of the A2780cp cells will be reversed through the Nrf2 siRNA transfection. The apoptosis rate of A2780cp_kd cell line was higher than the A2780cp cell group(p<0.05).Conclusion:The results of this study point out:The inhibitory effect of cisplatin to the growth of A2780cp and SKOV3 cell lines is not as significant as to the A2780 and Hey cell lines. The Nrf2 protein showed a high expression in ovarian cancer A2780cp and SKOV3 cell lines and a low expression in ovarian cancer A2780 and Hey cell lines.The Nrf2 protein can be interferenced through Nrf2 siRNA transfection. Thus, the chemoresistence of the A2780cp cells would be reversed.The summery is:the Nrf2 protein has a certain correlation with platinum-resistance in ovarian cancer cells.
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