| Objective:To observe the change of the plasma levels of Cholecystokinin (CCK) before and after fatty diet in patients with gallstone, and further to identify the value of plasma CCK for evaluating the motor function of gallbladderMethods:From June 2009 to April 2010,28 patients with gallstone confirmed by ultrasound examination were enrolled in this study, and 20 healthy subjects were taken as controls (group N).Gallbladder volumes were measured by Aloka 3500 B-type ultrasonography. All the subjects were studied after an overnight fast. Fast volume (FV) was measured before the fatty diet. The postprandial gallbladder volume was recorded at 30,60 min after fatty diet. Residual volume (RV) was defined as the smallest postprandial volume. Residual fraction (RF) was calculated with the formula (RV/FV×100%).According to the criterion promoted by Pomeranz and Shaffer, pathological contractor patients were defined by a RF exceeding the mean of controls added 2 SD.Then in the present study, patients with gallstone were subdivided into group S1 (patients with normal contractor,13 cases) and group S2 (patients with pathological contractor,15 cases). Meanwhile the concentrations of plasma CCK were measured by radioimmunoassay at the fast statue,30 and 60 min after fatty diet respectively Results:1.The plasma levels of CCK at the fast statue:there are no significant difference among these three groups (group N,2.52±0.53 pmol/1; group S1,3.98±1.52 pmol/1;group S2,3.29±1.09 pmol/1)2.The plasma levels of CCK after fatty diet:the levels of plasma CCK were elevated in all groups,and group S2 is more obvious than other two groups1).The plasma levels of CCK at 30 min after fatty diet:the level of group S2 (44.40±6.19 pmol/1) is significant higher than those in group N (12.38±1.89 pmol/1) and group S1 (13.77±2.00 pmol/1); but there is no significant difference between group N and group S1 (P>0.05)2).The plasma levels of CCK at 60 min after fatty diet:the level of group S2 (92.07±12.45 pmol/1) is significant higher than those in group N (25.16±2.07 pmol/1) and group S1 (26.26±2.01 pmol/1); but there is no significant difference between group N and group S1 (P>0.05)Conclusion:the postprandial concentration of plasma CCK has a negative correlation with the motor function of gallbladder. In patients with gallstone,if the motor function of gallbladder was normal,the concentration of plasma CCK could be in normal range. The results suggested that perhaps the levels of plasma CCK could be use to identify the motor function of gallbladder in patients with gallstone... |
