The Analysis Between Human-High-molecular-weight Salivary Mucin Content And Children's Caries Status

Purpose:Analysis the high molecular weight salivary mucin (MG1) content and the relationship between caries status of children through the experiment, in order to provide the basis for salivary mucin research and caries etiology of the information.Method: Randomly selected 305 children of aged 3 to 5 in two kindergartens of Changsha for the oral examination, according to the results divided into three groups:①no caries group:DMFT= 0;②low caries group:DMFT=1～4;③high caries group:DMFT≥5. By roughly the same number of age and gender randomly selected the three groups of all 20 children, test their caries activity with Resazurin disk diffusion method, and then took these children's plaque and saliva, analysis the MG1 content by ELISA kit, and the results were statistically analyzed.Results:①Resazurin paper method tests showed that the no caries group, low caries group and high caries group of children's caries activity significantly different (P<0.01).The caries activity of high caries group(DMFT≥5) children is higher than low caries group and non-caries group.②MG1 concentration in saliva:no caries group (DMFT= 0) MG1 average concentration of 13.63mg/L, low caries group (DMFT=1～4) MG1 average concentration of 12.96mg/L, high caries group (DMFT≥5) MG1 average concentration of 12.61mg/L. After statistical analysis, no caries group and high caries group MG1 concentration of saliva was significantly different (P<0.05), non-caries group and low-caries group concentration of saliva MG1 was no significant difference (P> 0.05), low-caries group and high-caries group concentration of saliva MG1 was no significant difference (P> 0.05). Male and female children MGl concentration in salivary was no significant difference (P> 0.05), the MG1 concentration of different age groups was no significant difference (P> 0.05).③MG1 content in plaque:test 10 groups MG1 plaque content, the outcome of the 2 groups was outside the scope of the test (less than minimum detectable concentration of 0.6 ng/mL), the average content of the remaining 8 groups MG1 was 0.123μg/g. As the 10 groups,2 groups were lower than the minimum detectable concentration, and we can not analysis the relationship between the caries status an MG1 content in the plaque.Conclusion:①Children whose DMFT≥5 caries activity was higher that of children chose DMFT<5.②The MG1 in saliva by Groups of children without caries were higher than that of high-caries group, MG1 concentration in saliva of children showed no significant correlation by gender and age.③The content of MG1 in plaque is about 0.123μg/g.