Association Analyses Of MtSNP 10398A/G And Risks To Ovarian And Cervical Cancers In Hunan Han Females

Our recent work had shown that the frequencies of the two alleles for mitochondrial DNA (mtDNA) single nucleotide polymorphisms (mtSNP) 10398A/G were comparable to each other in Hunan Han population (-45% and-55% respectively). It demonstrated that when association analyses were performed between mtDNA background and specific diseases, hunan Han population might have provided themselves as an ideal population for sample withdrawn. Based on recent reports on female-specific breast cancer risk with this mtSNP site, to explore its possible associations with ovarian and cervical cancer risks, molecular epidermiological investigation were performed in hunan Han females by means of control-case. Based on former protocol of amplification refractory mutation system (ARMS), improved real-time fluorescence mismatched-primer PCR was employed to individuall genetyping of mtSNP 10398A/G in cases groups of ovarian and cervical cancers. It was shown that there were 126 (54.5%) of mtSNP 10398A and 105 (45.5%) of G genotypes in the group of ovarian cancer and 136 (47.4%) for A and 151(52.6%) for G types in the cervical cancer group. The differences in allele frequencies were performed between case groups and the results of 219 10398A (45.3%) and 265 10398G (54.7%) genotypes for the control group of 484 healthy females which were performed earlierly. It were demonstrated here that, (1) a real-time fluorescence mismatched-primer PCR were set up for the first to rapidly and stably genotyping individuals at mtSNP 10398. (2) the frequency of mtSNP 10398A allele was statistically bigger than that in the case group of ovarian cancer (54.5% vs. 45.3%, P=0.020), which suggesting that hunan Han females of 10398A allele has a higher risk to developing ovarian cancer, comparing with that of a female of 10398G genotype. However, though a slightly raised ratio of mtSNP 10398A allele was also obtained from the case group of cervical cancer, there was no statistical significance comparing with the frequency in the control group (47.4% vs.52.6%, P=0.565) and thus, no definite association could be set up between this polymorphism and the risk of cervical cancer.