| BackgroundPrimary hepatocellular carcinoma (HCC) is one of the most common solid malgnancies worldwide which is also one of the high incidences of malignant tumors in our country. Despite improvements in surveillance, diagnosis and clinical treatment strategies in recent years, the prognosis of HCC is remain dismal. Invasion, metastasis and recurrences are the most cause of death and vital factors of clinical therapy and prognosis in HCC patients. Its occurrence, development and metastasis are complicated multifactors and multisteps process concerned with genetics and epigenetics. Therefore, it is of great significance to study the development, invasion and metastasis mechanisms of HCC. Current research in molecular biology, the metastasis-associated genes in HCC mainly are murine double minute 2 gene (MDM2), metastasis-associated tumor gene 1 (MTA-1), mammlian STE 20-like kinase 1 (MST1), etc; The invasion and metastasis-related protein are urokinase-type plasminogen activator uPA, matrix metalloproteinases MMPs, osteopontin OPN. Multiple factors result in tumor invasion and metastasis. It has been reported that overexpression of MTA-1and OPN is higher correlated with the tumor invasion, metastasis and poor prognosis in HCC, gastrointestinal carcinoma, breast carcinoma, but it has not been reported that the co-expression of MTA-1and OPN in the HCC correlated with the metastasis and prognosis.ObjectiveTo investigate the correlationship of the MTA-1, OPN expression in the early and late recurrence group with clinical pathological features and the early recurrence time after radical resection.MethodsImmunohistochemical method (SP method) was used to detect MTA-1, OPN expression in HCC tissues and the paratumoral tissues. The relationship between its expression and clinicopathological features and postoperative recurrence time were statistically analyzed in HCC patientsResults1. The positive expression of MTA-1 in HCC was 56.7% and in the paratumor tissues was 0 (p<0.05); OPN expression in HCC and paratumor tissues was 68.3% and 30% respectively (p<0.05). The positive expression of MTA-1 and OPN in the early recurrence group of HCC was higher than the later recurrence group.2. MTA-1 expression was not statistically related with gender, age, hepatitis B, serum AFP elevation while closely related with the tumor size, tumor number, tumor differentiation, clinical TNM stage and vascular invasion (p<0.05); OPN expression was not statistically associated with age, gender, serum AFP elevation, hepatitis B, tumor number, clinical TNM stage but correlated with tumor differentiation, tumor size and (p<0.05).3. MTA-1 expression is closely related with disease-free survival time. The medians for disease-free survival time in the positive expression group was 9 months, while the negative expression group was 17; The medians for disease-free survival time in OPN expression group was 10 months, while in the negative group was 18; Cumulative survival curves of the MTA-1+ group is significantly lower than the MTA-1- group (p＜0.05), The same with OPN. The expression of MTA-1, OPN in HCC predicts early recurrence. 4. The expression of MTA-1 and OPN was positively correlated in HCC.5. Tumor size, degree of differentiation, clinical stage, MTA-1 and OPN are independent promising predictors of tumor recurrence and disease-free survival time in HCC patients (p<0.05), while the MTA-1 and OPN are the major factors, r=5.739 (p<0.05).Conclusion:1. MTA-1, OPN is highly expressed in HCC, indicating that MTA-1, OPN may be associated with the occurrence and progress of HCC.2. The expression of MTA-1 and OPN may be associated with early recurrence after radical liver resection and can be served as predicators of tumor recurrence in hepatocellular carcinoma patients.3. The expression of MTA-1, OPN is positively correlated each other in HCC, suggesting that they have synergistic effect in the occurrence and progress of HCC.
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