Effects Of Electroacupuncture On Expression Of CRF Family And CRF Receptor1 In Traumatized Rats

Objective To explore the effects of electroacupunture (EA) on the abnormal hypothalamus-pituitary-adrenal axis (HPAA) in traumatized Rats, the expression of CRF peptides family and CRF receptor1 mRNA in hypothalamus was investigated.Methods Forty male Sprague-Dawley rats were randomly divided into four groups: intact group (INT, n=10); intact with EA group (INT+EA, n=10); surgical trauma group (ST, n=10); surgical trauma with EA group (ST+EA, n=10). Dorsomyotomy were performed on the rats under anesthesia. EA was administered via two stainless steel needles in two acupoints:"Zusanli" acupoint (ST36), and "Sanyinjiao" acupoint (SP6). The stimulation was generated by an EA apparatus (Hans) and lasted for 30 min. The stimulation parameters were 2mA of density and a frequency of 2/15 Hz. At the time of sacrifice, rats were decapitated and the trunk blood (1.0 ml) of four groups were collected respectively, The plasma was separated by centrifugation at 4℃and stored at-70℃until assayed. The brains were immediate removed and the hypothalamus were dissected and stored at-70℃. Concentration of ACTH, Cort, LH and T were determined by a double-antibody RIA. Total RNA was extracted using TRIzol Regent.Results The blood ACTH concentration decreased significantly in ST (P<0.05) compared with that in INT and INT+EA, and was higher in ST+ EA (P<0.05) than in ST. There were no disparities between INT and INT+EA. The blood concentration of Cort in ST group increased significantly (P<0.05) compared with that in INT and INT+EA, and was lower in ST+EA (P<0.05). While the concentration of LH and T did not change significantly between four groups. The expressions of hypothalamic CRFmRNA in the ST rats decreased significantly compared with those in the INT rats (P<0.05), and the level in the ST+EA (?) with tho(?) In the ST rats (P<0.05). However, no disparity was found between the levels in the INT and INT+EA rats. The expressions of hypothalamic UcnlmRNA in the ST rats increased significantly compared with those in the INT rats (P< 0.05), and the level in the ST+ EA decreased significantly compared with those in the ST rats (P<0.05). There were no significant changes in the expression of Ucn2mRNA, Ucn3mRNA and CRFR1mRNA among the four groups.Conclusion Based on above, EA on"Zusanli" and "Sanyinjiao" benefits the rats with surgical trauma-lead HPA disorders.