Cytochrome P450 ω-hydroxylase Inhibition Attenuates Isoprenaline-induced Apoptosis In Cultured Myocardiocytes

20-Hydroxyeicosatetraenoic acid (20-HETE), aω-hydroxylation product of arachidonic acid catalyzed by cytochrome P450 4A (CYP4A), plays a role in vascular smooth muscle remodeling and myocardial ischemia-reperfusion injury. Although its effects on angiogenic responses are known, it remains unclear whether 20-HETE acts on apoptosis of cardiomyocytes cells, an important step in cardiomyocytes cells injury remodeling, and what pathways are involved in the process.In this study, we inv estigated the effects of the selective CYPω-hydroxylase inhibitor on myocardial cells induced by Isoprenaline. Compared with vehicle group,17-odya significantly inhibited myocardial apoptosis as evidenced decreased by MTT assay, annexin-v FCM assay, mitochondrial potentials assay, positive nuclear staining and rt-pcr for bcl-2,bax. Compared with vehicle group, 20-HETE also can come to the same effect with group of Isoprenaline . Furthermore, 20-HETE induced the expression of bax, destroy the stability of mitochondria membrane.Such effects were reversed in the presence of 17-ODYA. Thus, these findings indicate that 17-odya protects rat myocardial cells against apoptosis by acting on mitochondrial potentials assay and which downstream proteinum.