Clinical Study On Detecting The MRNA Expression Of CK19 And Mammaglobin In Peripheral Blood Of Breast Cancer By Fq-pcr

ObjectiveTo detect and analyze the expression of cytokeratin-19 (CK19)and human mammaglobin (hMAM) mRNA in peripheral blood of breast cancer patients, to explore the correlation indicator to some extent could be used as parameters to predict prognosis and monitor recurrenceMethodFluorescence quantification-polymerase chain reaction (FQ-PCR) was used to detect the expressions of CK19 and hMAM mRNA in peripheral blood of 40 breast cancer patients and 10 healthy volunteers. Chi-square test was used for comparison between groups, and Kappa test was applied to analyze the correlation between CK19 and hMAM expressions.ResultsThe positive expression rates of CK10 and hMAM in breast cancer patients were 52.5%(21/40)and 55%(22/40), respectively, significantly higher than those of the healthy controls (P<0.05). There was a correlation between CK19 and hMAM expressions in the breast cancer patients (Kappa = 0.849,P=0.000). The positive expression of CK19 was correlated with tumor size, lymph node metastasis and progesterone receptor (PR) (P<0.05), not correlated with estrogen receptor (ER) and human epidermal growth factor receptor2 (HER-2). The positive expression of hMAM was correlated with tumor size, lymph node metastasis, ER and HER-2 (P<0.05), but was not correlated with PR.Conclusions Patients with breast cancer have notably higher CK19 in peripheral blood and hMAM expression than normal population, which reveals a significant correlation to the clinical parameters of breast cancer prognosis, such as tumor size, lymph node metastasis, and receptor status. Therefore, the correlation indicator to some extent could be used as parameters to predict prognosis and monitor recurrence...