| Backgroud: At present,the endometrium damage is one of the major problems which gynecologist encountered in clinical, for the treatment of endometrium damage the most important point in the treatment strategies is to promote regeneration and functional recovery of injuried endometrium, The current clinical regeneration treatment of the locally severe or extensive endometrium injury is still difficult to solve, which is the thorny issue of clinical treatment. In recent years with the study on theory of stem cell differentiation, more and more research on the clinical application of stem cells emerges in the academic reports,witch provides a new concept for the treatment of endometrial damage. Because the embryonic stem cells (ESC) have high regeneration and the potential to differentiate to other type of cells, so it is a good method to study injuried endometrium application of stem cells by transplanting a mumber of moese embryonic stem cells into the injuried endometrial lumina of the ICR mice, all the experimental research we did is to lay the foundation for the further study stem cell therapy for endometrial injury.Objective: The study was designed to study the mouse embryonic stem cells'intrauterine survival and migration after transplantation into the injuried endometrial lumina of ICR miceMethods:We cultured the EGFP+ES cells on the MEF feeder layer and maintained it in the undifferentiated state, detected its all-round identification by transplantating it into the kidney capsule, and tumor formation 4 weeks after transplantation, then analyzed it by HE staining and nestin,α-SMA and the AFP immunohistochemical staining;We make some endometrium injury of ICR mice models by thermal injury on tissue, and observe the situation of injuried endometrium in a series of time; then transplant a number of undifferentiated GFP+ embryonic stem cells(3-4×105)into the acute uterine cavity with acute injuried endometrium. Kill the mice and draw the uterine tissues in 7th day and 14th day after transplantation, observe the transplanted mouse embryonic stem cells'survival and migration directly by fluorescence microscopy and under the microscope after HE staining and Anti-GFP antibody immunohistochemical staining.Results: ES cells grew well on the MEF feeder cells as a clone-like, the EGFP- ES cells was observed expressing high green fluorescence under fluorescence microscope, and has teratoma formation with three germ layers markers expression (nestin,α-SMA, and AFP) when transplanted into the kidney capsule; we established mouse model with injuried endometrium successfully; We transplanted a number of EGFP- ES cells into the the uterine cavity of ICR mouse with acute injuried endometrium, we found that there was a lot of shedding tissue and no specific morphology of endometrial tissue in the cavity of uterus, no repair in the uterine wall by HE staining, also we could observe a cluster of positive round cells in the the cavity of uterine after anti-GFP antibody immunohistochemical staining 7 days later,but we have not observed into positive cells migrate to the uterine wall.Conclution:ES cells grows on the MEF feeder cells in the undifferentiated state and has all-round differentiation potential; establish the endometrial damage model by using the hot water's thermal effect on endometrium; when the undifferentiated mESC transplanted into the acute uterine cavity with injuried endometrium, mESC can survive in the uterine cavity。...
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