Identification Of Differentially Expressed Proteins In Dorsal Corticospinal Tract And Rubrospinal Tract Injured In The Rat

Objective:Through study the proteidic changes of Dorsal Corticospinal Tract and Rubrospinal Tract after spinal cord injury, we can lay a solid foundation for explore the restoration after spinal cord injury whether is consistent with the principles of the theory of evolution.Methods:we select for twenty 70±2 days,200-230g SD female rats. We randomly divided these rats into sham,dCST, FG+FC, RST injury group each group have 3 rats. Then we manufacture corresponding animal spinal cord damage model (dCST injury,RST injury and FG +FC injury). Three days after SCI, the rats were put to death and 1 cm tissue of spinal cord around the injured site were got out and weighed. The proteins from each group were separated by 2-DE. Lastly, the proteins were considered to be significantly regulatedthen followed by MS identification.Results:.The results of 2-DE following fluid percussion injury of astrocytes:1519±82,1492±94,1517±33,1378±112 protein spots were detected respectively in sham, hibateral RST, hibateral FG+hibateral FC, hibateral dCST injury group. Furthermore, most of these spots were dispersed into the scopes of pI5-7 and Mr10 kDa-80 kDa; The image of sham group was considered as the master image, the average match rate was more than 75%. Dynamic changes were identified and total 12 differentially expressed proteins were detected in hibateral RST injury group, which included 10 up-regulated spots, and 2 down-regulated spots. Dynamic changes were identified and total 12 differentially expressed proteins were detected in hibateral dCST injury group, which included 8 up-regulated spots, and 4down-regulated spots. Identification of the different displayed protein spots via MS analysis.Conclusion:According to the proteidic changes of Dorsal Corticospinal Tract and Rubrospinal Tract after spinal cord injury, we found the spinal cord recovery process in line with the principle of evolutionism.